(d) A heptapeptide was found to have an amino acid composition of Asp, Leu, Lys, Met, Phe and Tyr. Trypsin has no effect on the heptapeptide. One cycle of Edman degradation renders: CH2 NH Chymotrypsin treatment yields a dipeptide and a tetrapeptide, as well as a free amino acid. The tetrapeptide is known to contain Leu, Lys, and Met. Cyanogen bromide treatment generates a dipeptide, a tetrapeptide, and free Lys. Determine the amino acid sequence of the heptapeptide. Show how you obtain your answer.

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(d) A heptapeptide was found to have an amino acid composition of Asp, Leu, Lys, Met, Phe
and Tyr.
Trypsin has no effect on the heptapeptide.
One cycle of Edman degradation renders:
CH
NH
Chymotrypsin treatment yields a dipeptide and a tetrapeptide, as well as a free
amino acid. The tetrapeptide is known to contain Leu, Lys, and Met.
Cyanogen bromide treatment generates a dipeptide, a tetrapeptide, and free Lys.
Determine the amino acid sequence of the heptapeptide. Show how you obtain your answer.
Transcribed Image Text:(d) A heptapeptide was found to have an amino acid composition of Asp, Leu, Lys, Met, Phe and Tyr. Trypsin has no effect on the heptapeptide. One cycle of Edman degradation renders: CH NH Chymotrypsin treatment yields a dipeptide and a tetrapeptide, as well as a free amino acid. The tetrapeptide is known to contain Leu, Lys, and Met. Cyanogen bromide treatment generates a dipeptide, a tetrapeptide, and free Lys. Determine the amino acid sequence of the heptapeptide. Show how you obtain your answer.
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