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ction mutation in the SMNT gene. which of the following strategies would be suitable? Select all that apply Introduce a mutant human SMN1 allele as a transgene into mice, with transgene integration anywhere in the mouse genome; breed the resulting transgenics to generate homozygotes Use CRISPR-Cas9 to introduce frameshift mutations in the mouse SMN1 gene; breed the resulting mutant mice to generate homozygous mutants Use CRISPR-Cas9 technology to cut the mouse SMN1 gene; supply a mutant human SMN1 allele as a repair O template and select for transgenics where the the human allele replaced the mouse allele of SMN1; breed the resulting mutant mice to generate homozygous mutants Introduce a wild type (functional) human SMN1 allele as a transgene into mice; select transgenics where the human gene replaced the wild type mouse SMN1 gene via homologous recombination

ction mutation in the SMNT gene. which of the following strategies would be suitable? Select all that apply Introduce a mutant human SMN1 allele as a transgene into mice, with transgene integration anywhere in the mouse genome; breed the resulting transgenics to generate homozygotes Use CRISPR-Cas9 to introduce frameshift mutations in the mouse SMN1 gene; breed the resulting mutant mice to generate homozygous mutants Use CRISPR-Cas9 technology to cut the mouse SMN1 gene; supply a mutant human SMN1 allele as a repair O template and select for transgenics where the the human allele replaced the mouse allele of SMN1; breed the resulting mutant mice to generate homozygous mutants Introduce a wild type (functional) human SMN1 allele as a transgene into mice; select transgenics where the human gene replaced the wild type mouse SMN1 gene via homologous recombination

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You would like to create a mouse model for spinal muscular atrophy, a recessive human disease caused by a loss of function mutation in the SMN1 gene. Which of the following strategies would be suitable? Select all that apply Introduce a mutant human SMN1 allele as a transgene into mice, with transgene integration anywhere in the mouse genome; breed the resulting transgenics to generate homozygotes Use CRISPR-Cas9 to introduce frameshift mutations in the mouse SMN1 gene; breed the resulting mutant mice to generate homozygous mutants Use CRISPR-Cas9 technology to cut the mouse SMN1 gene; supply a mutant human SMN1 allele as a repair template and select for transgenics where the the human allele replaced the mouse allele of SMN1; breed the resulting mutant mice to generate homozygous mutants Introduce a wild type (functional) human SMN1 allele as a transgene into mice; select transgenics where the human gene replaced the wild type mouse SMN1 gene via homologous recombination
Transcribed Image Text:You would like to create a mouse model for spinal muscular atrophy, a recessive human disease caused by a loss of function mutation in the SMN1 gene. Which of the following strategies would be suitable? Select all that apply Introduce a mutant human SMN1 allele as a transgene into mice, with transgene integration anywhere in the mouse genome; breed the resulting transgenics to generate homozygotes Use CRISPR-Cas9 to introduce frameshift mutations in the mouse SMN1 gene; breed the resulting mutant mice to generate homozygous mutants Use CRISPR-Cas9 technology to cut the mouse SMN1 gene; supply a mutant human SMN1 allele as a repair template and select for transgenics where the the human allele replaced the mouse allele of SMN1; breed the resulting mutant mice to generate homozygous mutants Introduce a wild type (functional) human SMN1 allele as a transgene into mice; select transgenics where the human gene replaced the wild type mouse SMN1 gene via homologous recombination
You are interested in studying the FBN1 gene, a candidate identified via GWAS for a gene involved in heritable risk of heart disease. You have a human cardiac myocyte cell line with wild type alleles of FBN1, and plan to use the CRISPR/Cas9 system to make various edits to the FBN1 gene to determine their phenotypic effects on cellular functions. After directing Cas9 cleavage to the FBN1 gene with an appropriate guide RNA, you will utilize two different repair systems to generate different types of FBN1 mutations. Sort the items listed below into the repair categories provided based on their relationship to generation of FBN1 mutations by that repair mechanism. Introduction of a specific mutation into the FBN1 gene Frameshift mutation Insertion mutation Nonhomologous end joining (NHEJ) Repair template Deletion mutation Trimming off or filling in unpaired nucleotides created by a jagged double-strand DNA break Homology-directed repair (HDR)
Transcribed Image Text:You are interested in studying the FBN1 gene, a candidate identified via GWAS for a gene involved in heritable risk of heart disease. You have a human cardiac myocyte cell line with wild type alleles of FBN1, and plan to use the CRISPR/Cas9 system to make various edits to the FBN1 gene to determine their phenotypic effects on cellular functions. After directing Cas9 cleavage to the FBN1 gene with an appropriate guide RNA, you will utilize two different repair systems to generate different types of FBN1 mutations. Sort the items listed below into the repair categories provided based on their relationship to generation of FBN1 mutations by that repair mechanism. Introduction of a specific mutation into the FBN1 gene Frameshift mutation Insertion mutation Nonhomologous end joining (NHEJ) Repair template Deletion mutation Trimming off or filling in unpaired nucleotides created by a jagged double-strand DNA break Homology-directed repair (HDR)
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