Homework Help is Here – Start Your Trial Now!
Science
Biology
Create a schematic diagram of the procedures below

Create a schematic diagram of the procedures below

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
icon
Concept explainers
Question

Create a schematic diagram of the procedures below

 

(Also attached below is an example of a schematic diagram)

Group I (n=10) Aluminium chloride (AC) Prepared 40 specimens of teeth with flattened dentin surface embedded in cold cure acrylic ↓ Group II (n=10) Ferric sulphate (FS) Group III (n=10) Tannic acid (TA) The hemostatic agent applied for 5 minutes ។ Group IV (n=10) Control group Rinsed with distilled water and gently air dried for 3-5 seconds Self-adhesive resin cement placed into a 2X2 mm dimension matrix attached to the dentinal surface Polymerized with visible light curing unit for 30 seconds Shear bond strength tested using an Instron universal testing machine
Transcribed Image Text:Group I (n=10) Aluminium chloride (AC) Prepared 40 specimens of teeth with flattened dentin surface embedded in cold cure acrylic ↓ Group II (n=10) Ferric sulphate (FS) Group III (n=10) Tannic acid (TA) The hemostatic agent applied for 5 minutes ។ Group IV (n=10) Control group Rinsed with distilled water and gently air dried for 3-5 seconds Self-adhesive resin cement placed into a 2X2 mm dimension matrix attached to the dentinal surface Polymerized with visible light curing unit for 30 seconds Shear bond strength tested using an Instron universal testing machine
C. Spectrophotometric Measurements of the Hill Reaction 1. Place 15 mL of the 0.05-mg chlorophyll/ mL suspension in a test tube. Wrap the tube with aluminum foil and chill in an ice bucket. This is the source of unheated chloroplasts. 2. Place the remaining 10 mL of the 0.05-mg chlorophyll/mL suspension in a test tube and heat until boiling using an alcohol lamp. Heating inactivates the chloroplasts. Stand the heated mixture for 5 min. to allow the debris to settle. 3. Prepare the contents of each setup listed in Table 5.1 in clean test tubes. Pipet contents in each test tube in the order given. For the "Unheated (Dark)" setup, immediately wrap the test tube with aluminum foil even before the first reading is made. 4. Your instructor alu Upcialing a WIII demonstrate the nandling of a cur spectrophotometer. Set the spectrophotometer at 605 nm wavelength. Zero the spectrophotometer using the appropriate blank. Use the "Heated Blank" for "Heated" experimental tube, and use the "Unheated Blank" for both "Unheated (Light)", and "Unheated (Dark)" experimental tubes. 5. Add 1 mL 0.1% DPIP to experimental tubes "Heated", "Unheated (Light)", and "Unheated (Dark)" prior to the initial readings. DPIP must be added to experimental tubes when about to be placed in spectrophomoter. Cover the mouth of the test tube with a piece of aluminum foil and the thumb and invert the test tube several times to mix its contents. Pour the solution in a clean cuvette. Do not delay reading if DPIP was already mixed. 6. Take the initial readings of the experimental set-ups "Heated", "Unheated (Light)", and "Unheated (Dark)". For the "Unheated (Dark)" setup, immediately wrap the test tube in aluminum foil after taking the initial reading. Measure the absorbance again after 30 min. Uncover only upon reading the absorbance. 7. After the initial readings, place all the test tubes in a beaker placed in an ice bath and illuminate the test tubes with a 100-watt bulb placed 18 inches away. Measure the absorbance of the "Heated" and the "Unheated (Light)" tubes at 5-min. intervals up to 30 min. Mix the contents before reading.
Transcribed Image Text:C. Spectrophotometric Measurements of the Hill Reaction 1. Place 15 mL of the 0.05-mg chlorophyll/ mL suspension in a test tube. Wrap the tube with aluminum foil and chill in an ice bucket. This is the source of unheated chloroplasts. 2. Place the remaining 10 mL of the 0.05-mg chlorophyll/mL suspension in a test tube and heat until boiling using an alcohol lamp. Heating inactivates the chloroplasts. Stand the heated mixture for 5 min. to allow the debris to settle. 3. Prepare the contents of each setup listed in Table 5.1 in clean test tubes. Pipet contents in each test tube in the order given. For the "Unheated (Dark)" setup, immediately wrap the test tube with aluminum foil even before the first reading is made. 4. Your instructor alu Upcialing a WIII demonstrate the nandling of a cur spectrophotometer. Set the spectrophotometer at 605 nm wavelength. Zero the spectrophotometer using the appropriate blank. Use the "Heated Blank" for "Heated" experimental tube, and use the "Unheated Blank" for both "Unheated (Light)", and "Unheated (Dark)" experimental tubes. 5. Add 1 mL 0.1% DPIP to experimental tubes "Heated", "Unheated (Light)", and "Unheated (Dark)" prior to the initial readings. DPIP must be added to experimental tubes when about to be placed in spectrophomoter. Cover the mouth of the test tube with a piece of aluminum foil and the thumb and invert the test tube several times to mix its contents. Pour the solution in a clean cuvette. Do not delay reading if DPIP was already mixed. 6. Take the initial readings of the experimental set-ups "Heated", "Unheated (Light)", and "Unheated (Dark)". For the "Unheated (Dark)" setup, immediately wrap the test tube in aluminum foil after taking the initial reading. Measure the absorbance again after 30 min. Uncover only upon reading the absorbance. 7. After the initial readings, place all the test tubes in a beaker placed in an ice bath and illuminate the test tubes with a 100-watt bulb placed 18 inches away. Measure the absorbance of the "Heated" and the "Unheated (Light)" tubes at 5-min. intervals up to 30 min. Mix the contents before reading.
Expert Solution
steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Health assessment
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS