Answered: Create a flow chart on how to prepare a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Create a flow chart on how to prepare a nutrient agar

Transcribed Image Text:B. Preparation of Nutrient Agar (NA) a. To every liter of nutrient broth, add 15 – 20 g agar. b. Dissolve the agar in a boiling water bath. After the agar has dissolved, restore to original volume by adding hot water. Agar is a component used for solidifying bacteriological media; it is a gel-forming polysaccharide, which is extractable from several species of red seaweeds (agarophytes). It is a mixture of at least 2 polysaccharides: agarose and agaropectin, with components. Agar should be capable of remaining molten and fluid at 40 - 45°C after cooling from the boiling point. Grades are available and depending upon brand, the required amount of agar will vary. Agar slopes or slants are made by allowing liquefied agar to solidify in slanting position. Media should always be stored in cool and moist environment to prevent evaporation.

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