NB = HA = HB 1.5199 ×104 4.8796 x10 -4 1.973 x10-3 The number of theoretical plates for the column is calculated as N = N. X NB A Using NĄ and NB, calculate the expected resolution for this separation. resolution: mm mm Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at 1.205 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? TRA tR.B KA KB = = α = 11.425 12.035 NA = 9.481 What is the separation factor (relative retention), a, for this separation? 9.987 1.053 The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the number of theoretical plates, N, and plate height, H, for each compound. 6.1480 x104 min min
NB = HA = HB 1.5199 ×104 4.8796 x10 -4 1.973 x10-3 The number of theoretical plates for the column is calculated as N = N. X NB A Using NĄ and NB, calculate the expected resolution for this separation. resolution: mm mm Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at 1.205 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? TRA tR.B KA KB = = α = 11.425 12.035 NA = 9.481 What is the separation factor (relative retention), a, for this separation? 9.987 1.053 The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the number of theoretical plates, N, and plate height, H, for each compound. 6.1480 x104 min min
Chapter15: Electrophoresis And Other Separation Methods
Section: Chapter Questions
Problem 5P
Related questions
Question
everything is correct, I just need help with the last unanswered question
![NB =
HA =
HB
1.5199 ×104
4.8796 x10
-4
1.973 x10-3
The number of theoretical plates for the column is calculated as
N = N. X NB
A
Using NĄ and NB, calculate the expected resolution for this separation.
resolution:
mm
mm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1548810f-0d74-4833-a3a0-92b9ff116e67%2F5e8f72f9-3988-4600-aa30-5e86a66ca30c%2Fuveqq9w_processed.png&w=3840&q=75)
Transcribed Image Text:NB =
HA =
HB
1.5199 ×104
4.8796 x10
-4
1.973 x10-3
The number of theoretical plates for the column is calculated as
N = N. X NB
A
Using NĄ and NB, calculate the expected resolution for this separation.
resolution:
mm
mm
![Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick
stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at
1.205 min.
What are the adjusted retention times, t, and retention factors, k, for compounds A and B?
TRA
tR.B
KA
KB
=
=
α =
11.425
12.035
NA =
9.481
What is the separation factor (relative retention), a, for this separation?
9.987
1.053
The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the
number of theoretical plates, N, and plate height, H, for each compound.
6.1480 x104
min
min](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1548810f-0d74-4833-a3a0-92b9ff116e67%2F5e8f72f9-3988-4600-aa30-5e86a66ca30c%2Fqmvxu8_processed.png&w=3840&q=75)
Transcribed Image Text:Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick
stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at
1.205 min.
What are the adjusted retention times, t, and retention factors, k, for compounds A and B?
TRA
tR.B
KA
KB
=
=
α =
11.425
12.035
NA =
9.481
What is the separation factor (relative retention), a, for this separation?
9.987
1.053
The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the
number of theoretical plates, N, and plate height, H, for each compound.
6.1480 x104
min
min
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