NB = HA = HB 1.5199 ×104 4.8796 x10 -4 1.973 x10-3 The number of theoretical plates for the column is calculated as N = N. X NB A Using NĄ and NB, calculate the expected resolution for this separation. resolution: mm mm Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at 1.205 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? TRA tR.B KA KB = = α = 11.425 12.035 NA = 9.481 What is the separation factor (relative retention), a, for this separation? 9.987 1.053 The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the number of theoretical plates, N, and plate height, H, for each compound. 6.1480 x104 min min

NB = HA = HB 1.5199 ×104 4.8796 x10 -4 1.973 x10-3 The number of theoretical plates for the column is calculated as N = N. X NB A Using NĄ and NB, calculate the expected resolution for this separation. resolution: mm mm Consider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at 1.205 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? TRA tR.B KA KB = = α = 11.425 12.035 NA = 9.481 What is the separation factor (relative retention), a, for this separation? 9.987 1.053 The widths at half-height, w₁/2, of the peaks for compounds A and B are 0.120 min and 0.253 min, respectively. Find the number of theoretical plates, N, and plate height, H, for each compound. 6.1480 x104 min min

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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