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Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Isoelectric Point Problem A mixture of two proteins are to be separated by isoelectric focusing Isoelectric Molecular Point (pl) Weight G3P Dehydrogenase (GDH) 8.3 36.0 DNA Ligase (Lig) 6.0 60.0 Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field. In which direction will each protein in the table migrate at pH 7.0 Protein Name A A. Both GDH and Lig will have a negative (-) charge and travel towards the positive (+) terminal OB. Both GDH and Lig will have a positive (+) charge and travel towards the negative (-) terminal OC. GDH will have a positive (+) charge and travel towards the negative (-) terminal Lig will have a negative (-) charge and travel towards the positive (+) terminal OD. GDH will have a negative (-) charge and travel towards the positive (+) terminal Lig will have a positive (+) charge and travel towards the negative (-) terminal
Transcribed Image Text:Isoelectric Point Problem A mixture of two proteins are to be separated by isoelectric focusing Isoelectric Molecular Point (pl) Weight G3P Dehydrogenase (GDH) 8.3 36.0 DNA Ligase (Lig) 6.0 60.0 Both protein samples are placed into a gel with a constant pH of 7.0. The gel is then placed into an electric field. In which direction will each protein in the table migrate at pH 7.0 Protein Name A A. Both GDH and Lig will have a negative (-) charge and travel towards the positive (+) terminal OB. Both GDH and Lig will have a positive (+) charge and travel towards the negative (-) terminal OC. GDH will have a positive (+) charge and travel towards the negative (-) terminal Lig will have a negative (-) charge and travel towards the positive (+) terminal OD. GDH will have a negative (-) charge and travel towards the positive (+) terminal Lig will have a positive (+) charge and travel towards the negative (-) terminal
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