Ch 3. Two mobile phases were evaluated to study the behavior of five dyes. The following data from each chromatogram were collected: CPLOWET oite Owaiva Mobile Phase 1: Acetone/Water Mobile Phase 2: Ethanol/Water Distance RF Distance RF traveled (cm) noina traveled (cm) Solvent front 4.7 4.9 Green dye Red dye Yellow dye Blue dye Purple dye ,१४on .76cm Tocm .53em .29cm 2.9 .62 cm 4.8 1.3 3.7 4.0 0.8 2.9 .62(m 2.6 3.1 1.4 a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. b) Explain your reasons for choosing which solvent would be most effective in separating the green from the blue dye. olduloz esal c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.

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Explain your reasons for choosing which solvent would be most effective in separating the green from blue dye.

Ch
3. Two mobile phases were evaluated to study the behavior of five dyes. The following
data from each chromatogram were collected:
CPLOWET
oite
Owaiva
Mobile Phase 1: Acetone/Water
Mobile Phase 2: Ethanol/Water
Distance
RF
Distance
RF
traveled (cm)
noina
traveled (cm)
Solvent front
4.7
4.9
Green dye
Red dye
Yellow dye
Blue dye
Purple dye
,१४on
.76cm
Tocm
.53em
.29cm
2.9
.62 cm
4.8
1.3
3.7
4.0
0.8
2.9
.62(m
2.6
3.1
1.4
a) Calculate the RF values for each dye for both chromatographic systems. Record these
values in the table above.
Transcribed Image Text:Ch 3. Two mobile phases were evaluated to study the behavior of five dyes. The following data from each chromatogram were collected: CPLOWET oite Owaiva Mobile Phase 1: Acetone/Water Mobile Phase 2: Ethanol/Water Distance RF Distance RF traveled (cm) noina traveled (cm) Solvent front 4.7 4.9 Green dye Red dye Yellow dye Blue dye Purple dye ,१४on .76cm Tocm .53em .29cm 2.9 .62 cm 4.8 1.3 3.7 4.0 0.8 2.9 .62(m 2.6 3.1 1.4 a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above.
a) Calculate the RF values for each dye for both chromatographic systems. Record these
values in the table above.
b) Explain your reasons for choosing which solvent would be most effective in
separating the green from the blue dye.
olduloz esal
c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.
Transcribed Image Text:a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. b) Explain your reasons for choosing which solvent would be most effective in separating the green from the blue dye. olduloz esal c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.
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