catalyzes the reaction

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. The enzyme lactate dehydrogenase catalyzes the reaction below.
NADH
+ H*
NAD+
OH
Pyruvate
Lactate
NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic
spectrum, but NAD' does not.
a. Assuming that you have a spectrophotometer that can measure light at this
wavelength, qualitatively sketch a plot of A, 340 nm VS. time.
How does this plot compare to the one from the experiment on the alkaline
phosphatase-catalyzed reaction of p-nitrophenylphosphate?
b. Would you use a highly accurate pH meter to monitor the progress of the pyruvate
reaction? Why or why not?
Transcribed Image Text:1. The enzyme lactate dehydrogenase catalyzes the reaction below. NADH + H* NAD+ OH Pyruvate Lactate NADH absorbs light at 340 nm in the near-ultraviolet region of the electromagnetic spectrum, but NAD' does not. a. Assuming that you have a spectrophotometer that can measure light at this wavelength, qualitatively sketch a plot of A, 340 nm VS. time. How does this plot compare to the one from the experiment on the alkaline phosphatase-catalyzed reaction of p-nitrophenylphosphate? b. Would you use a highly accurate pH meter to monitor the progress of the pyruvate reaction? Why or why not?
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