Case Study #5 Setting: Fann Teaching Hospital in Dakar, Senegal. Subject: 12-year old boy who was hospitalized for acute meningoencephalitis. The CT scan was normal, however, study of the cerebrospinal fluid revealed cytological and biochemical abnormalities. A sample of the CSF was sent to the hospital lab for analysis. The results follow... Key to Media Used in Case Studies (shown in picture from left to right. I have given you the answers if they are negative or positive based on the picture.) Phenol Red Glucose (It's positive) Phenol Red Sucrose (Positive) Results holds for lactose too Phenol Red Xylose (positive) SIM (negative) Read as Sulfur / Indole Lysine Decarboxylase (negative) Ornithine Decarboxylase (Positive) Methyl Red (positive) Vogues-Proskauer (negative) Urea (Positive) Simmon’s Citrate (positive) Nutrient Gelatin (it is clearly still solid, therefore negative) Results of Inoculated Media Based on the results of the media shown above, determine the causative agent of the infection. Use the figures in this presentation or in your lab manual for identification. “B” for this organism is its Phenol Red – Mannitol result
Case Study #5
Setting: Fann Teaching Hospital in Dakar, Senegal.
Subject: 12-year old boy who was hospitalized for acute meningoencephalitis. The CT scan was normal, however, study of the cerebrospinal fluid revealed cytological and biochemical abnormalities.
A sample of the CSF was sent to the hospital lab for analysis.
The results follow...
Key to Media Used in Case Studies (shown in picture from left to right. I have given you the answers if they are negative or positive based on the picture.)
- Phenol Red Glucose (It's positive)
- Phenol Red Sucrose (Positive)
- Results holds for lactose too
- Phenol Red Xylose (positive)
- SIM (negative)
- Read as Sulfur / Indole
- Lysine Decarboxylase (negative)
- Ornithine Decarboxylase (Positive)
- Methyl Red (positive)
- Vogues-Proskauer (negative)
- Urea (Positive)
- Simmon’s Citrate (positive)
- Nutrient Gelatin (it is clearly still solid, therefore negative)
Results of Inoculated Media
Based on the results of the media shown above, determine the causative agent of the infection. Use the figures in this presentation or in your lab manual for identification.
“B” for this organism is its Phenol Red – Mannitol result
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