Calculate the [OH-] and the pH of a solution with [H] = 3.0 x 10 Mat 25 °C. [OH-] = [H*] = Calculate the [H*] and the pOH of a solution with [OH-] = 2.1 x 10 M at 25 °C. [H*] = Calculate [H*] and [OH-] of a solution with pH= 5.70 at 25 °C. M R-NH;=R-NH₂ + H* In what pH range can glycine be used as an effective buffer due to its amino group? M How much 5 M KOH must be added to 1.0 L of 0.1 M glycine at pH 9.0 to bring its pH to 10.0? pH = pOH = The amino acid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine, which has a pK, of 9.6, can exist either in the protonated form-NH; or as the free base -NH₂, because of the reversible equilibrium M [OH-] = M In a 0.1 M solution of glycine at pH 9.0, what fraction of glycine has its amino group in the -NH; form? When 99% of the glycine is in its -NH; form, what is the numerical relation between the pH of the solution and the pK, of the amino group? A compound has a pK, of 7.4. You add 100 mL of a 1.0 M solution of this compound at pH 8.0 to 30 mL of 1.0 M hydrochloric acid. What is the pH of the resulting solution? Calculate the pH of a dilute solution that contains a molar ratio of sodium acetate to acetic acid (pK₁ = 4.76) of 5:1. Calculate the concentrations of acetic acid (pK, = 4.76) and sodium acetate necessary to prepare a 0.6 M buffer solution at pH 6.0. A buffer contains 0.020 mol of lactic acid (pK, = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C Calculate the change in pH after adding 6.5 mL of 0.90 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 6.5 mL of 0.90 M HCl to 1.0 L of pure water. Calculate the hydrogen ion concentration of solution #1, which has pH 2.75. O Na+ Calculate the hydrogen ion concentration of solution #2, which has pH 7.75. Calculate the hydrogen ion concentration of solution # 3, which has pH 11.50. buffer pH: buffer pH change: water pH change: The quality assurance lab just received a shipment of three solutions. The lab manager asks you to verify the stock solutions and label each bottle with the solution's hydrogen ion concentration, [H*]. You first use the pH meter determine the pH of each solution. [H*] = [H*] = units [H+] = units M M M