Calculate the number of kcal and amount of protein provided by the following formula: 500 mL of 50% dextrose and 1 liter of a 10% amino acid solution. If 250 mL of a 20% intravenous fat emulsion were added, how many more kcal would be provided?
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- Pb(Ac)2: Add 5 mL NaOH and a few crystal of Pb( Ac)2 to 2-3 mL protein suspension. Heat in a boiling water bath for 5 - 10 minutes with occasional mixing of the content of the tube. Describe the color change. what will be the color reaction of intact protein (Table) Gluten l Bean Protein l Casein Pb(ac)29. A daily diet of a 55-ycar-old woman, consisted of a 500 g of carbohydrates, 100 g of animal fats and 150 g of proteins, at the background of low physical activity. What possible consequences for the lipid metabolism such lifestyle could trigger? For the answer: a) compare the daily food consumption of the patient with the norm for this age and level of physical activity; b) draw the scheme of TAG synthesis in the liver and continue with their following transport to the tissues; c) draw the scheme of metabolic pathways which are more active in adipocytes of this patient compared with an individual of the same age consuming balanced dict; d) name the biologically active molecules released by adipocytes of this patient.6a. Using your 5 mg/mL stock solution, you wish to make 100µL of the correct concentration of protein to load. How do you make this working solution? (Yes- you would want this to be in 1x sample buffer, but that makes this question more complicated. So for this question just make the protein in water.) Volume of 10mg/ml Final Volume ! 1 100μL of protein 6b. This is a 1: A N Body* @ 2 W S dilution. #3 X E D $ 4 A C R F B I % 5 T V MacBook Pro U וכ H E U 8 N J ( 0
- 1. A 50-years-old man consumes daily about 90 g of fat of animal origin (fatty meat, butter, cheese), another man of the same age - 50 g of fat of animal origin and 30 g of plant oil. Which food is healthier? For the answer: a) name the differences in fatty acid composition of these TAGS; b) draw the structures of the TAG molecules specific to animal fat solid at room temperature and to olive oil liquid at the same temperature; c) explain which TAGS are more useful. urine diffo4. The table below describes a procedure in preparing standard solutions of a protein sample with different concentrations. (A) Determine the concentrations of each tube if the starting concentration of the protein is 500 mg ml (B) Determine the equation of the line if the concentration (x-axis) was plotted vs the absorbance (y-axis). (C) What is the concentration, in mg ml of the diluted unknown protein sample if the absorbance at 595 nm is 0.333? Tube Volume of protein, Volume of water, Final concentration, Absorbance mL mL mg ml [at 595 nm) 1 0.00 5.00 0.113 2 0.70 4.30 0.184 3 1.40 3.80 0.251 14 2.10 2.90 0.329 2.80 2.20 0.385 3.50 1.50 0.454 7 4.20 0.80 0.503 5.00 0.00 0.5778. For protein adsorption to the types of material surfaces designated below, indicate whether the adsorption process is primarily mediated by either enthalpic or entropic effects. Briefly justify your answers for each case. (A) A material surface functionalized with nonpolar groups (i.e., hydrophobic). (B) A material surface functionalized with polar groups (i.e., neutral hydrophilic). (C) A material surface functionalized with charged groups (i.e., +/-).
- g) Describe the biological function of the peptide hormone insulin. h) Haemoglobin is an example of a quaternary protein. Briefly describe the structure of this protein and state its biological function.1. Since the pK values for aspartic acid are 2.0, 3.9 and 10.0, it follows that the isoelectric (pH) is (A) 3.0 (B) 3.9 (C) 5.9 (D) 6.0Given the Ramachandran Plot below, identify the protein components that could adopt the phi-psi angle combination indicated by the number 3. (IF YOU COPY UR ANSWER FROM ANOTHER WEBSITE I WILL DISLIKE)
- 8. A dipeptide T, obtained from the breakdown of an enzyme found in the human body, is made up of Amino Acids R and S. (a) Classify Amino Acids R and S as an acidic amino acid or a basic amino acid. (b) Name Bond Q. (c) State any ONE type of protein that could be found in human body. (2%) (1%) (1%)1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:Define and give description of the importance of the isoelectric precipitation of proteins. (Hint: Isoelectric Precipitation of Proteins-Casein from Milk )