Calculate the K₁ value for the inhibitor shown below in the presence of 5.0 µM inhibitor. Use 2 significant figures. Velocity (nmol-min ¹) K₁ = 250 200 150 100 50 i 1 2 3 4 5 7 Substrate concentration (MM) No inhibitor μM 6 With inhibitor 8 9 10

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.