calculate the ALT concentration level in serum if the absorbance of the sample after 1 minute was 0.2 and after 2 minute was 0.25 and after 3 minute was 0.3 and the molar absorbance (ɛ) of NADH at 340 nm is 6300, the lightpath (I) is 1 cm, the total reaction volume (Vt) is 1.05 at 37°C, the sample volume (Vs) is 0.05mL ?

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