C. Suppose the section of interphase chromatin described above came from an active gene in a muscle cell. This section (with its necklace like structure) is most likely to be derived from: (the core promoter) (a proximal regulatory element of the gene) (an enhancer of the gene) (an exon in the gene) (an intron in the gene) (any of the cis-acting elements listed) (any transcribed region of the gene) (any of these) (none of these). Explain briefly how you arrived at your choice.

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Chapter1: The Human Body: An Orientation
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4-4. A. You isolate chromatin from an interphase cell and treat it with a small amount of DNAse. You spread out the
treated chromatin, stain it, and look at it in the electron microscope. You see a structure that looks like a piece of a
necklace, with 4 beads.
A-1. The number of molecules of double stranded DNA in the structure should be:
(1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (0) (>10).
A-2. The longest double stranded DNA molecule in the structure should be:
(200 bp) (400 bp) (600 bp) (800 bp) (1000 bp) (1200 bp) (1600 bp) (2000 bp) (<200 bp) (> 2000 bp)
(none of these – there's no DNA in the structure).
A-3. The number of molecules of H2A in the structure should be:
(1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (12) (15) (16) (20) (24)(0) (>24).
Explain your answers to part A. (A labeled picture is fine.) Show how you arrived at all 3 answers.
B. Suppose you could examine the equivalent section of the chromatin when the cell is in mitosis. (Equivalent =
contains same DNA as that in the chromatin described above.)
B-1. Then this section of the chromatin would be (shorter) (about the same length) (shorter or longer)
(longer) (same or shorter) (same or longer) (any of these).
molecules of H1.
B-2. This section of the chromatin in mitosis should contain
B-3. The number of nucleosomes in this section of the chromosome in mitosis would be (higher) (lower)
(the same) (higher or the same) (lower or the same) than the number in interphase.
Explain briefly how this section of chromatin in mitosis compares to the equivalent section in interphase.
C. Suppose the section of interphase chromatin described above came from an active gene in a muscle cell.
This section (with its necklace like structure) is most likely to be derived from:
(the core promoter) (a proximal regulatory element of the gene) (an enhancer of the gene) (an exon in the gene)
(an intron in the gene) (any of the cis-acting elements listed) (any transcribed region of the gene)
(any of these) (none of these). Explain briefly how you arrived at your choice.
Transcribed Image Text:4-4. A. You isolate chromatin from an interphase cell and treat it with a small amount of DNAse. You spread out the treated chromatin, stain it, and look at it in the electron microscope. You see a structure that looks like a piece of a necklace, with 4 beads. A-1. The number of molecules of double stranded DNA in the structure should be: (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (0) (>10). A-2. The longest double stranded DNA molecule in the structure should be: (200 bp) (400 bp) (600 bp) (800 bp) (1000 bp) (1200 bp) (1600 bp) (2000 bp) (<200 bp) (> 2000 bp) (none of these – there's no DNA in the structure). A-3. The number of molecules of H2A in the structure should be: (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (12) (15) (16) (20) (24)(0) (>24). Explain your answers to part A. (A labeled picture is fine.) Show how you arrived at all 3 answers. B. Suppose you could examine the equivalent section of the chromatin when the cell is in mitosis. (Equivalent = contains same DNA as that in the chromatin described above.) B-1. Then this section of the chromatin would be (shorter) (about the same length) (shorter or longer) (longer) (same or shorter) (same or longer) (any of these). molecules of H1. B-2. This section of the chromatin in mitosis should contain B-3. The number of nucleosomes in this section of the chromosome in mitosis would be (higher) (lower) (the same) (higher or the same) (lower or the same) than the number in interphase. Explain briefly how this section of chromatin in mitosis compares to the equivalent section in interphase. C. Suppose the section of interphase chromatin described above came from an active gene in a muscle cell. This section (with its necklace like structure) is most likely to be derived from: (the core promoter) (a proximal regulatory element of the gene) (an enhancer of the gene) (an exon in the gene) (an intron in the gene) (any of the cis-acting elements listed) (any transcribed region of the gene) (any of these) (none of these). Explain briefly how you arrived at your choice.
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