C. How much does DNA of a WBC weigh? (Note that WBCs are diploid.) d. How much DNA can you collect from 10 mL of whole blood? (Assume that there is no loss during DNA extraction.)
C. How much does DNA of a WBC weigh? (Note that WBCs are diploid.) d. How much DNA can you collect from 10 mL of whole blood? (Assume that there is no loss during DNA extraction.)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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can you help with 1c and 1d please
Transcribed Image Text:1. You are extracting DNA from 10 mL of whole blood. WBC's (white blood cells) are collected via
centrifugation. WBC concentration is 8 x 106 cells / mL. Answer the following questions.
a. How many WBC's can you collect from the 10 mL of whole blood?
g
b. The human genome (haploid) size is 3 x 10º base pairs (bp). The molecular weight of DNA is 660
How much does one copy of human genome weigh? (HINT: How many molecules are
mol x bp
there in one mole of a substance?):
C.
How much does DNA of a WBC weigh? (Note that WBCs are diploid.)
d. How much DNA can you collect from 10 mL of whole blood? (Assume that there is no loss
during DNA extraction.)
e. The purification of DNA allows scientists to perform many powerful genetic techniques in the
fields of medicine and forensics. Describe at least two different techniques that can be performed
once DNA has been isolated.
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