C. b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant --- dsdor Elephant 1 Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 Elephant 10 FH71 62 64 62 62 62 64 62 64 62 64 64 64 60 62 62 62 62 62 62 FH19 FH60 FH67 97 189 147 193 147 97 185 147 95 193 147 103 183 147 95 185 147 97 193 147 91 193 147 105 187 145 95 187 147 95 187 145 91 193 147 97 193 149 97 193 151 97 193 147 97 193 147 103 193 147 97 193 147 97 189 147 95 193 147 105 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

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Please solve question b) and c) * The seized tusks came for elephant #8.
### Analysis of a Seized Elephant Tusk

You are investigating a newly seized tusk and want to determine if it belongs to one of the elephants recently found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is provided alongside genotypes from 10 elephants.

### Genotype Data and Analysis

**Table 1:** Genotype for five STR loci from a seized elephant tusk.

- **Seized Tusk:**
  - **FH19**: 193
  - **FH60**: 147
  - **FH67**: 97
  - **FH71**: 62
  - **FH129**: 152

**Table 2:** Genotypes using five STR loci for 10 slaughtered elephants.

The elephants in Table 2 have different genotypes at these loci, which we need to compare with the seized tusk to find a match.

#### a. Identifying the Source Elephant

- **Question:** Did the seized tusk come from one of the recently slaughtered elephants?
- **Answer:** Yes, the seized tusk matches with Elephant 8. The alleles for all STR loci of Elephant 8 correspond perfectly with those of the seized tusk.

#### b. Band Pattern Illustration

- **Task:** Draw the expected pattern of bands for each STR locus.
  - STR loci have distinct allele lengths that correspond to band positions.
  - Include a DNA ladder with base pairs at 75, 125, 150, and 175 for reference.

**Diagram Explanation:**

1. **DNA Ladder Bands:** 
   - Represents standard DNA fragments for size comparison.
2. **Seized Tusk Bands:**
   - Bands at positions corresponding to alleles 193, 147, 97, 62, and 152.
3. **Matching Elephant Bands:**
   - Aligns exactly with the seized tusk, showing identical allele sizes.

#### c. Conclusion from Gel Pattern

The band pattern of the seized tusk and Elephant 8 are identical, confirming that the seized tusk came from Elephant 8. This match supports the conclusion based on DNA profiling, ensuring the uniqueness of DNA fragments and accurately identifying the source.
Transcribed Image Text:### Analysis of a Seized Elephant Tusk You are investigating a newly seized tusk and want to determine if it belongs to one of the elephants recently found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is provided alongside genotypes from 10 elephants. ### Genotype Data and Analysis **Table 1:** Genotype for five STR loci from a seized elephant tusk. - **Seized Tusk:** - **FH19**: 193 - **FH60**: 147 - **FH67**: 97 - **FH71**: 62 - **FH129**: 152 **Table 2:** Genotypes using five STR loci for 10 slaughtered elephants. The elephants in Table 2 have different genotypes at these loci, which we need to compare with the seized tusk to find a match. #### a. Identifying the Source Elephant - **Question:** Did the seized tusk come from one of the recently slaughtered elephants? - **Answer:** Yes, the seized tusk matches with Elephant 8. The alleles for all STR loci of Elephant 8 correspond perfectly with those of the seized tusk. #### b. Band Pattern Illustration - **Task:** Draw the expected pattern of bands for each STR locus. - STR loci have distinct allele lengths that correspond to band positions. - Include a DNA ladder with base pairs at 75, 125, 150, and 175 for reference. **Diagram Explanation:** 1. **DNA Ladder Bands:** - Represents standard DNA fragments for size comparison. 2. **Seized Tusk Bands:** - Bands at positions corresponding to alleles 193, 147, 97, 62, and 152. 3. **Matching Elephant Bands:** - Aligns exactly with the seized tusk, showing identical allele sizes. #### c. Conclusion from Gel Pattern The band pattern of the seized tusk and Elephant 8 are identical, confirming that the seized tusk came from Elephant 8. This match supports the conclusion based on DNA profiling, ensuring the uniqueness of DNA fragments and accurately identifying the source.
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