c) Calculate the transmittance given that the absorbance is 0.279. d) Calculate transmittance given that the percent transmittance is 68.2%. e) Calculate percent transmittance given that the absorptivity is 15623 L/mol cm, pathlength is 1.00 cm, and the concentration is 5.33 x 10 M.

If a solution has an absorbance value of 0.518 at a wavelength of 460 nm, what is the percent transmittance of this solution at this wavelength?

Calculate the absorbance for a solution containing a colored species with a percent transmittance (%T) of 58.23.

What value of absorbance corresponds to 45.0% T? If a 0.010 M solution exhibits 45.0% T at some wavelength what will be the percent transmittance for a 0.020 M solution of the same substance?

Indicate the relationship of proportionality that exists between: a) concentration and absorbance b) absorbance and transmittance

2. Calculate the absorbance value (A) for a solution that absorbs 89.5% of the light striking it. (Hint: What is the percent transmittance? Review the background reading)

3. You have two solutions containing the same compound having the same pink color. The concentration of the first solution is 2.0 M, while the concentration of the other solution is 2.5 M. Absorbance of each solution was measured with the same spectrophotometer at the same wavelength. a) Which solution has the more intense pink color? b) Which solution has the higher absorbance?

A solution with a known concentration equal to 3.4 mM gave an absorbance reading of 0.820 in a spectrophotometer. Assuming that there is a linear relationship between concentration and absorbance up to a concentration of 4.0 mM, what would the absorbance reading be for a solution with a concentration 2.2 mM?

Spectrophotometry and Beer's Law 4. A solution has 23.6% transmittance in a cell with a path length of 1.00 cm. If the material responsible for the absorption of light has a molar absorptivity e = 36.2 (L/mole-cm) at the wavelength used, calculate the concentration of this solution in mole/L. (Show all work.) M

A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A.  52 B.  3.1 C.  2.4 D.  0.52 E.  0.031