Bromothymol blue (MWt 624 g/mole, weak acid, pka 7.1) diluted solution is prepared by diluting 2.5 mL of 0.04 % w/v Bromothymol blue stock solution to final volume of 10 mL using 0.02 M Phosphate buffer pH 9 (pka 7.2). 1) Calculate the dilution factor and the Bromothymol blue 1) mg/mL of the diluted solution 2) molarity of the diluted solution 2) Calculate the % ionization of the diluted Bromothymol blue 3) What is the likely color of the solution when Bromothymol blue ionized is blue and the Bromothymol blue non-ionized form is yellow?

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Question 1
Bromothymol blue (MWt = 624 g/mole, weak acid, pka 7.1) diluted solution is prepared by diluting 2.5
mL of 0.04 % w/v Bromothymol blue stock solution to final volume of 10 mL using 0.02 M Phosphate
buffer pH 9 (pka 7.2).
1) Calculate the dilution factor and the Bromothymol blue 1) mg/mL of the diluted solution 2)
molarity of the diluted solution
2) Calculate the % ionization of the diluted Bromothymol blue
3) What is the likely color of the solution when Bromothymol blue ionized is blue and the Bromothymol
blue non-ionized form is yellow?
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Transcribed Image Text:Question 1 Bromothymol blue (MWt = 624 g/mole, weak acid, pka 7.1) diluted solution is prepared by diluting 2.5 mL of 0.04 % w/v Bromothymol blue stock solution to final volume of 10 mL using 0.02 M Phosphate buffer pH 9 (pka 7.2). 1) Calculate the dilution factor and the Bromothymol blue 1) mg/mL of the diluted solution 2) molarity of the diluted solution 2) Calculate the % ionization of the diluted Bromothymol blue 3) What is the likely color of the solution when Bromothymol blue ionized is blue and the Bromothymol blue non-ionized form is yellow? Edit View Insert Format Tools Table Paragraph v BIU A 2 T?v:
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