4. Inhibition of Carbonic Anhydrase by Acetazolamide Carbonic anhydrase is strongly inhibited by the drug acetazolamide which is used as a diuretic (increases the production of urine) and to treat glaucoma (reduces excessive high pressure within the eyeball). Carbonic anhydrase plays an important role in these and other secretory processes, because it participates in regulating the pH and bicarbonate content of many body fluids. The experimental curve of reaction velocity (given here as a percentage of Vmax) versus [S] for the carbonic anhydrase reaction is illustrated below (upper curve). When the experiment is repeated in the presence of acetazolamide, the lower curve is obtained. From an inspection of the curves and your knowledge of the kinetic properties of competitive and noncompetitive enzyme inhibitors, determine the nature of the inhibition by acetazolamide. Explain. 100 No inhibitor 50 Acetazolamide 2 4 8. 10 100 [S] (*"A JO %) A
4. Inhibition of Carbonic Anhydrase by Acetazolamide Carbonic anhydrase is strongly inhibited by the drug acetazolamide which is used as a diuretic (increases the production of urine) and to treat glaucoma (reduces excessive high pressure within the eyeball). Carbonic anhydrase plays an important role in these and other secretory processes, because it participates in regulating the pH and bicarbonate content of many body fluids. The experimental curve of reaction velocity (given here as a percentage of Vmax) versus [S] for the carbonic anhydrase reaction is illustrated below (upper curve). When the experiment is repeated in the presence of acetazolamide, the lower curve is obtained. From an inspection of the curves and your knowledge of the kinetic properties of competitive and noncompetitive enzyme inhibitors, determine the nature of the inhibition by acetazolamide. Explain. 100 No inhibitor 50 Acetazolamide 2 4 8. 10 100 [S] (*"A JO %) A
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Help with #4 thanks
![4. Inhibition of Carbonic Anhydrase by Acetazolamide
Carbonic anhydrase is strongly inhibited by the drug acetazolamide which is used as a diuretic
(increases the production of urine) and to treat glaucoma (reduces excessive high pressure within
the eyeball). Carbonic anhydrase plays an important role in these and other secretory processes,
because it participates in regulating the pH and bicarbonate content of many body fluids. The
experimental curve of reaction velocity (given here as a percentage of Vmax) versus [S] for the
carbonic anhydrase reaction is illustrated below (upper curve). When the experiment is repeated
in the presence of acetazolamide, the lower curve is obtained. From an inspection of the curves
and your knowledge of the kinetic properties of competitive and noncompetitive enzyme
inhibitors, determine the nature of the inhibition by acetazolamide. Explain.
100
No inhibitor
50
Acetazolamide
2
4
8.
10
100
[S]
(*"A JO %) A](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F85793e9a-8859-44de-af1b-868e70709835%2Fd0af2a3b-00a5-4d27-bf77-37425ae9b8b0%2F8vmrm8c_processed.jpeg&w=3840&q=75)
Transcribed Image Text:4. Inhibition of Carbonic Anhydrase by Acetazolamide
Carbonic anhydrase is strongly inhibited by the drug acetazolamide which is used as a diuretic
(increases the production of urine) and to treat glaucoma (reduces excessive high pressure within
the eyeball). Carbonic anhydrase plays an important role in these and other secretory processes,
because it participates in regulating the pH and bicarbonate content of many body fluids. The
experimental curve of reaction velocity (given here as a percentage of Vmax) versus [S] for the
carbonic anhydrase reaction is illustrated below (upper curve). When the experiment is repeated
in the presence of acetazolamide, the lower curve is obtained. From an inspection of the curves
and your knowledge of the kinetic properties of competitive and noncompetitive enzyme
inhibitors, determine the nature of the inhibition by acetazolamide. Explain.
100
No inhibitor
50
Acetazolamide
2
4
8.
10
100
[S]
(*"A JO %) A
Expert Solution
Step 1
Enzymes are proteins that aid in the speeding up of metabolic reactions. Certain chemicals can slow down enzyme-catalyzed processes. These compounds are known as inhibitors or enzyme inhibitors. They can block an enzyme from conducting its normal catalytic function by adhering to it.
Trending now
This is a popular solution!
Step by step
Solved in 4 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON