Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme. Test Unknown Isolate B Luminescence Cell Shape Gram Staining Oxygen Requirement Motility Drop) Positive Straight Rods Negative Facultative Anaerobe 2 3 4 (Hanging Motile Growth at Negative 0% NaCi 3% NaC1 Positive 6% NaCl Positive Accumulation of PHB Flagella Oxidase Positive 1 Polar 10 11 12 Positive Nitrate Reduction Negative Positive 13 Gelatinase Growth at 35°C Growth at 4°C Methyl Red Voges-Proskauer Catalase Starch Hydrolysis Lysine Decarboxylase Indole Production Utilization of 14 15 Negative Positive 16 17 Positive 18 Positive Negative Negative Negative 19 20 21 22 23 Glucose Positive (fermentative) Positive (fermentative) Negative Negative Positive (fermentative) Negative Positive (oxidative) 24 Lactose 25 Mannitol 26 Sucrose 27 Xylose Inositol 28 29 Galactose

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Below are morphological, physiological and biochemical characteristics of an
unknown luminous bacterium isolated from the gills of a marine fish. Use the data
below to identify the isolate. Make an identification scheme.
Test
Unknown Isolate B
Luminescence
Cell Shape
Gram Staining
Oxygen Requirement
Motility
Drop)
Growth at
Positive
Straight Rods
Negative
Facultative Anaerobe
3
4
5
(Hanging
Motile
Negative
0% NaCi
7
3% NaC1
Positive
8
6% NaC1
Positive
Accumulation of PHB
Flagella
Oxidase
Positive
1 Polar
Positive
9
10
11
12
Nitrate Reduction
13
Gelatinase
Negative
Positive
Growth at 35°C
Growth at 4°C
14
15
Negative
Positive
Methyl Red
Voges-Proskauer
Catalase
Starch Hydrolysis
Lysine Decarboxylase
Indole Production
Utilization of
Glucose
Lactose
Mannitol
16
17
Positive
18
Positive
19
Negative
Negative
Negative
20
21
22
Positive (fermentative)
Positive (fermentative)
Negative
Negative
Positive (fermentative)
Negative
Positive (oxidative)
23
24
25
26
Sucrose
27
Xylose
Inositol
28
29
Galactose
Transcribed Image Text:Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme. Test Unknown Isolate B Luminescence Cell Shape Gram Staining Oxygen Requirement Motility Drop) Growth at Positive Straight Rods Negative Facultative Anaerobe 3 4 5 (Hanging Motile Negative 0% NaCi 7 3% NaC1 Positive 8 6% NaC1 Positive Accumulation of PHB Flagella Oxidase Positive 1 Polar Positive 9 10 11 12 Nitrate Reduction 13 Gelatinase Negative Positive Growth at 35°C Growth at 4°C 14 15 Negative Positive Methyl Red Voges-Proskauer Catalase Starch Hydrolysis Lysine Decarboxylase Indole Production Utilization of Glucose Lactose Mannitol 16 17 Positive 18 Positive 19 Negative Negative Negative 20 21 22 Positive (fermentative) Positive (fermentative) Negative Negative Positive (fermentative) Negative Positive (oxidative) 23 24 25 26 Sucrose 27 Xylose Inositol 28 29 Galactose
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