Bacteriophage Lamda Describe what method use to retrieved gene of interest from the plaques.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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Bacteriophage Lamda
Describe what method use to retrieved gene of interest from the plaques.
Transcribed Image Text:Bacteriophage Lamda Describe what method use to retrieved gene of interest from the plaques.
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So the lambda phage lacks a contractile tail, it is unable to 'push' its DNA through a bacterial cell membrane during an infection event. Having evolved the tip of its tail to interact with a particular pore to allow access of its DNA to the hosts, it must instead employ an already-existing pathway to invade the host cell.In order to categorise phage transcriptional regulatory systems, phage RNAP is either present or absent. Without endogenous RNAP, phages like T4 and exploit the host's RNAP to control the expression of their own genes. We'll go through how these phages produced these incredibly effective and complex regulatory systems in the next sections.

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