A trisaccharide was isolated from a new species of red algae. The sample was hydrolyzed and monosaccharide units were analyzed in different colorimetric tests. Based on the data below, what are the identity of monosaccharide A, B, and C?
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A trisaccharide was isolated from a new species of red algae. The sample was hydrolyzed and monosaccharide units were analyzed in different colorimetric tests. Based on the data below, what are the identity of monosaccharide A, B, and C?
A= fructose, B=mannose, C=xylose |
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A= xylose, B=mannose, C= fructose |
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A = mannose, B= fructose, C=xylose |
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A= xylose, B= fructose, C=galactose |
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A=galactose, B= fructose, C=xylose |
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A=xylose, B= fructose, C=mannose |
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A= fructose, B= xylose, C=galactose |
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A=galactose, B=xylose, C=fructose |
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- Test Done and Result Carbohydrate Identity Molisch test Seliwanoff's test Benedict's test Barfoed's test Lugol's Iodine Orcinol's Test Ozazone test test Brown solution Light blue solution No crystalization Violet Ring Light yellow solution Light blue solution Blue solution Green solution Red solution in 1 Brownish Violet Ring Cherry red solution Light blue solution No crystalization min solution Brown solution Cherry red solution in 15 min Light blue solution Brownish Violet Ring Brick red ppt No crystalization| solution Red solution Red solution in 1 min Brownish Violet Ring Light yellow solution Brick red ppt crystals structure solution Red solution Light blue solution Brownish Violet Ring Light yellow solution Brick red ppt crystal structure solution Blue solution Red solution in 1 Brownish Violet ring Light yellow solution Light blue solution Crystal srtructure min solutionEosin Y test solution procedure in standardizationGiven Test Reagent Positive or Negative Result Reason/Explanation Lime Soda Molisch Test Sucrose Fehling’s Test Fructose Tollen’s Test
- Given Test Reagent Positive or Negative Result Reason/Explanation Ribose Bial’s Orcinol Test Maltose Benedict’s Test Milk Molisch Test Cellulose Iodine TestGiven Test Reagent Positive or Negative Result Reason/Explanation Galactose Nylander’s Test Cellobiose Iodine Test Glucose Seliwanoff’s TestEosin Y Test solution procedure in preparation
- Positive or Given Test Reagent Result Reason/Explanation Negative Lime Soda Molisch Test Sucrose Fehling's Test Fructose Tollen's Test Ribose Bial's Orcinol Test Maltose Benedict's Test Milk Molisch Test Cellulose lodine TestExperimental Procedures 1. Obtain five unknown lipid samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh samples for each qualitative test. 3. For copper acetate test, add 2 ml of the test reagent to the sample and heat in a water bath for 3-5 minutes. Observe and record the color change. 4. For acrolein test, add 2 scoops of KHSO4 to the sample using a spatula. Heat the samples vigorously through a Bunsen burner or alcohol lamp for 3-5 minutes. Carefully smell the sample afterwards by wafting of the vapor to your nose. A sharp irritating odor indicates a positive reaction. 5. For Hubl's test, add 5 drops of the test reagent to the sample and shake the mixture thoroughly for 3 minutes. Observe and record the color change. 6. For ammonium molybdate test, add 2 ml of concentrated nitric acid and 5 drops of ammonium molybdate reagent to the sample. Heat the mixture for 3-5 minutes in a water bath. Observe for formation of…Chemical Test Just fill out Table 2 by writing “+” if the compound is soluble/miscible in the solvent. Otherwise, write “-“. Table 2. Data on the results of chemical tests for C4H6O2. Chemical Test Observations + or - Ninhydrin test no colored spots on filter paper iodoform test clear pale-yellow solution Br2/CH2Cl2 (dark) clear red-orange solution Molisch test turbid colorless solution Tollen’s Test clear, colorless solution
- option isoelectric focusinggel filtrationsalting outdifferential centrifugationEdman degradationion exchangedialysisCheck which test (s) will yield a positive result or negative result for the given compounds. Sample Biuret Test Ninhydrin test Hopkin's cole test Xanthoproteic test Albumin Gelatin Casein Tyrosine Glycine Ala-Gly Cys-Leu His-Gly-Tyr Gly-Gly-Met Beef StockHonorSociety org č. * SpiasiLa * Maps New Tab Describe your color observations of the Nitrate test. a. after adding Reagents A, B (and/or znic): cements b. Did the organism reduce nitrate? (yes or no) ments c. Is the final product nitrate, nitrite or ammonia/nitrogen gas? sions ing es Question 13 4 pts y Resources ules Based on your observations of the SIM test: ple a. Did sulfur reduction occur (yes/no)? zes b. Did the organism produce the enzyme tryptophanase (yes/no)? abus m c. Is the organism positive or negative for the motility test?