at is the wavelength of maximum aborbance for the Bradford reagent in its blue form. at is the wavelength of maximum aborbance for the Bradford reagent in its red form. at is the wavelength of maximum sensitivity for the spectrophotometer. doesn't really matter. Any wavelength would work fine.
at is the wavelength of maximum aborbance for the Bradford reagent in its blue form. at is the wavelength of maximum aborbance for the Bradford reagent in its red form. at is the wavelength of maximum sensitivity for the spectrophotometer. doesn't really matter. Any wavelength would work fine.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Transcribed Image Text:Why do you measure the absorbance at 595 nm when do in the Bradford assay?
That is the wavelength of maximum aborbance for the Bradford reagent in its blue form.
That is the wavelength of maximum aborbance for the Bradford reagent in its red form.
That is the wavelength of maximum sensitivity for the spectrophotometer.
O It doesn't really matter. Any wavelength would work fine.
Expert Solution
Step 1
Bradford reagent in Bradford assay is used to to assess the amount of protein in a sample. The reagent binds to proteins, and the binding causes the dye to undergo a color change in the visible spectrum, with the absorbance maximum moving from 470 to 595 nm.
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