As an intern in a research laboratory, you were asked to assist a researcher working on the characterization of pathogens found in a domestic wastewater sample. Specifically, you were tasked to prepare 350mL of a solution that will be used to resuspend and extract specific components from the pelleted slew of pathogens. The extraction solution is composed of 0.75 M phosphate buffer pH 8.0 (Ka2 = 6.2 × 10–8), 1.25 M CaCl2, 0.5% (w/v) SDS (sodium dodecyl sulfate), and 2.5mg per mL proteinase K. The following solutions/reagents are available in the lab in sufficient amounts: 5.0 N CaCl2 solution SDS powder (MM=288.38 g/mol) 20mg/mL proteinase K stock solution 3N HCl and 2M NaOH solutions solid NaH2PO4 (MM=119.98 g/mol) and Na2HPO4 (MM=141.96 g/mol) Calculate the amount of reagents needed in order to prepare the desired components of the extraction solution. Show complete calculations and box your final answers.
PREPARATION OF CHEMICAL REAGENTS
As an intern in a research laboratory, you were asked to assist a researcher working on the characterization of pathogens found in a domestic wastewater sample. Specifically, you were tasked to prepare 350mL of a solution that will be used to resuspend and extract specific components from the pelleted slew of pathogens. The extraction solution is composed of 0.75 M phosphate buffer pH 8.0 (Ka2 = 6.2 × 10–8), 1.25 M CaCl2, 0.5% (w/v) SDS (sodium dodecyl sulfate), and 2.5mg per mL proteinase K.
The following solutions/reagents are available in the lab in sufficient amounts:
5.0 N CaCl2 solution
SDS powder (MM=288.38 g/mol)
20mg/mL proteinase K stock solution
3N HCl and 2M NaOH solutions
solid NaH2PO4 (MM=119.98 g/mol) and Na2HPO4 (MM=141.96 g/mol)
Calculate the amount of reagents needed in order to prepare the desired components of the extraction solution. Show complete calculations and box your final answers.
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