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![A band of solute is applied to the inlet of the chromatography column shown. Match the shapes of the peaks to the
corresponding locations of the band in the column as it moves through the column.
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- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?
- Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.You need to administer furosemide (Lasix) 2 mg/minute via continuous IV infusion on a controller. Pharmacy has sent a bag of Lasix 400 mg diluted in D5W 250 mL. How many milliliters per hour will you set on the controller? Fill in the blanks. Answer: ml/hr
- Using a diagrammatical presentation show how serial dilution was performed if the final volume in each tube was 5ml.The Doctor ordered 900 ml of dextrose water to be infused over 2 hours by infusion pump set at a drop factor of 60. Calculate the amount of dextrose water (in ml) the patient should receive every 45 minutes. Round to the nearest mililiter.The physician ordered 700ml of a special electrolyte solution to flow at 75 gtt/min. The drop factor is 15 gtt/ml. How long will it take in hours and minutes will it take to infuse? using dimensional analysis and show work
- In the preparation of an intravenous infusion, a vial containing 100 mg of drug is diluted to 2 mL with sodium chloride for injection. Then, the contents of the vial are added to 100 mL of an infusion solution. Calculate the final strength of the drug infusion in percentage strength (% w/v)You receive a medication order to prepare an IV bag for infusion. You are required to add 30 mEq of potassium acetate to the IV bag. On hand, you have a vial of potassium acetate with a strength of 2 mEq/mL. What volume from the vial will you draw in order to prepare the IV bag?Indicate the quantity and unit of measure where specified.Calculate the final dilution in the following dilution series: a. 1 mL in 9 mL dilution & 100uL spread onto the plate b. 1 mL in 9 mL dilution followed by a 100ul in 900ul dilution & 100uL spread onto the plate (note bacteria are counted on plates as cfu/mL)