Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
An in vitro experiment used the isotope 14C-acetyl-CoA to identify 14C- oxaloacetate (OAA) as the final product of the citrate cycle. Explain why 14C OAA could not be found when the reaction lacked P₁?

An in vitro experiment used the isotope 14C-acetyl-CoA to identify 14C- oxaloacetate (OAA) as the final product of the citrate cycle. Explain why 14C OAA could not be found when the reaction lacked P₁?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
13
An in vitro experiment used the isotope 14C-acetyl-CoA to identify 14C- oxaloacetate (OAA) as the final product of the citrate cycle. Explain why 14C OAA could not be found when the reaction lacked P₁? Pi is required in the malate dehydrogenase reaction, which was inhibited, so no 14C-OAA is made. Pi is a required in the succinyl-CoA synthetase reaction, which was inhibited, so no 14C-OAA is made. Succinyl-CoA synthetase uses P; to generate alpha-ketoglutarate, so no 14C-OAA is made. Succinyl-CoA synthetase requires ADP + P; to form GMP, so without P₁, no 14C-OAA is made. The P₁ is an activator of citrate lyase in the cytosol, so without P₁, so no 14C-OAA is made.
Transcribed Image Text:An in vitro experiment used the isotope 14C-acetyl-CoA to identify 14C- oxaloacetate (OAA) as the final product of the citrate cycle. Explain why 14C OAA could not be found when the reaction lacked P₁? Pi is required in the malate dehydrogenase reaction, which was inhibited, so no 14C-OAA is made. Pi is a required in the succinyl-CoA synthetase reaction, which was inhibited, so no 14C-OAA is made. Succinyl-CoA synthetase uses P; to generate alpha-ketoglutarate, so no 14C-OAA is made. Succinyl-CoA synthetase requires ADP + P; to form GMP, so without P₁, no 14C-OAA is made. The P₁ is an activator of citrate lyase in the cytosol, so without P₁, so no 14C-OAA is made.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS