Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV.
b.These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV.
- Scanning power objective = 5X;
- Low power objective = 40X;
- High power objective = 100X;
- Eyepiece = 10X;
- Low power field of view (FOV) = 1.5 mm
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