Alternative splicing helps to explain O why there are fewer MRNAS than there are human genes. O why different proteins can be made from the same genes. O the great variety in protein modifications. O the large number of genes in the human genome. O the origins of microRNAs.

Alternative splicing helps to explain O why there are fewer MRNAS than there are human genes. O why different proteins can be made from the same genes. O the great variety in protein modifications. O the large number of genes in the human genome. O the origins of microRNAs.

Name: Is this correct Alternative splicing helps to explainO why there are f
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Description: Is this correct Alternative splicing helps to explainO why there are fewer MRNAS than there are human genes.O why different proteins can be made from the same genes.O the great variety in protein modifications.O t he large number of genes in the huma

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 11P: Post-transcriptional Modification of Eukaryotic mRNAs What might be the advantages of capping,...

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In vitro translation systems have been developed inwhich specific RNA molecules can be added to a testtube containing a bacterial cell extract that includes allthe components needed for translation (ribosomes,tRNAs, amino acids). If a radioactively labeled aminoacid is included, any protein translated from that RNAcan be detected and displayed on a gel. If a eukaryoticmRNA is added to the test tube, would radioactive protein be produced? Explain.
What is the phenomenon of RNA interference (RNAi)? DuringRNAi, explain how the double-stranded RNA is processed andhow it leads to the silencing of a complementary mRNA.
What is the benefit of alternative splicing? O The polarity of a protein can be adjusted for other circumstances O The protein sequence can be altered to adjust functionality O The protein can be linearized or made circular to accommodate locale O The length of a protein can be adjusted to meet other needs
Scientists have exploited the siRNA pathway toperform a technique called RNA interference—ameans to knock down the expression of a specificgene without having to make mutations in it. Theidea is to introduce dsRNA corresponding to thetarget gene into an organism; the dsRNA is thenprocessed into an siRNA that leads to the degradation of the target gene’s mRNA. One clever methodfor delivery of the dsRNA to some organisms (thenematode C. elegans, for example) is to feed thembacteria transformed with a recombinant plasmidthat expresses dsRNA.a. Draw a gene construct that, when expressed from aplasmid in bacteria, could be used to knock downby RNA interference the expression of gene X ofC. elegans.b. How can you test if gene X expression is obliterated in worms that have eaten the bacteria transformed with a plasmid containing your construct?c. Do you think that only gene X expression will beaffected in these worms? Explain.
In Figure 8-15, what do you think would be the effect ofa G to A mutation in the first G residue of the intron?
Explain well asap
If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense mutation cause a protein to be shorter, longer, or the same length as the normal 700 amino acid proteins? What would be the effect of a nonsense mutation? A sense mutation?
The Events in Transcription Initiation Describe the sequence of events involved in the initiation of transcription by E. coil RNA polymerase. Include in your description those features a gene must have for proper recognition and transcription by RNA poIymerase.
The human α-tropomyosin gene is alternativelyspliced to produce different forms of α-tropomyosinmRNA in different cell types (Figure Q6–3). For all forms ofthe mRNA, the protein sequences encoded by exon 1 arethe same, as are the protein sequences encoded by exon10. Exons 2 and 3 are alternative exons used in differentmRNAs, as are exons 7 and 8. Which of the following state-ments about exons 2 and 3 is the most accurate? Is thatstatement also the most accurate one for exons 7 and 8?Explain your answers.A. Exons 2 and 3 must have the same number ofnucleotides.B. Exons 2 and 3 must each contain an integral num-ber of codons (that is, the number of nucleotides dividedby 3 must be an integer).C. Exons 2 and 3 must each contain a number ofnucleotides that when divided by 3 leaves the sameremainder (that is, 0, 1, or 2).
Explain how RNA splicing and alternative splicing are relevant to your life and how is it useful? Need help with this question, thank you!
How would you describe the "purpose" of alternative splicing? Alternative RNA splicing... is a common feature of prokaryotic gene expression O b. is a mechanism for increasing the rate of transcription O c. is due to the absence of particular snRNPs d. results in the production of different versions of proteins from a single gene O e. results in the production of similar proteins from different genes
Both eukaryotic and prokaryotic promoters O contain enhancer or silencer sequences. O contain TATA boxes. O are important for telling RNA polymerase where to bind. O determine the reading frame of a gene.