Q4,5 A. Your laboratory requires you to look into the gene identified from Salmonella entericasubsp. enterica serovar Typhimurium str. LT2. The gene has been deposited in NCBI andannotated as STM3777 cytoplasmic protein [ Salmonella enterica subsp. enterica serovarTyphimurium str. LT2 ]; GenBank: AE006468.2.1. Where is this gene location in the bacterial genome?2. What is the length of the gene?3. Obtain the FASTA format of this gene sequence.4. Translate the nucleotide sequence to protein sequence.5. Can you postulate the function of this gene using bioinformatics tool? Try both nucleotideblast and protein blast.

Answered: A. Your laboratory requires you to look…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Q4,5

A. Your laboratory requires you to look into the gene identified from Salmonella enterica
subsp. enterica serovar Typhimurium str. LT2. The gene has been deposited in NCBI and
annotated as STM3777 cytoplasmic protein [ Salmonella enterica subsp. enterica serovar
Typhimurium str. LT2 ]; GenBank: AE006468.2.
1. Where is this gene location in the bacterial genome?
2. What is the length of the gene?
3. Obtain the FASTA format of this gene sequence.
4. Translate the nucleotide sequence to protein sequence.
5. Can you postulate the function of this gene using bioinformatics tool? Try both nucleotide
blast and protein blast.

Expert Solution

Step 1

Salmonella enterica subsp. enterica is a subspecies of Salmonella enterica, the rod-shaped, flagellated, aerobic, Gram-negative bacterium. Many of the pathogenic serovars of the S. enterica species are in this subspecies, including that responsible for typhoid.

Salmonella enterica subspecies enterica serovar Typhi (S. Typhi), a bacterium with a genome size of around 4.8 Mbp in length and containing at least 4900 annotated genes .

The gene sspH2 is located in the vicinity of the tRNAProL in the S. enterica serovar Typhi and S. enterica serovar Typhimurium chromosomes.

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