a. What is the probability that their child will not be affected by this trait? b. A geneticist uses RFLP analysis (described in Tool Box 3.1), in combination with a polar body assay, to determine if their child is likely to be affected by this trait. In this analysis, the size of the band on the gel represents a chromosome that has either the "normal" allele or the "disease" allele. The gel is shown in Figure Q6.5B, with each lane of the gel showing the results for one member of her family. The lane labeled "PB"is the analysis of the first polar body. Based on this analysis, what is the probability that their child will not be affected by this trait?

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I am unsure about both parts.

**Looking back 6.17**

The woman II-2 shown in the pedigree in Figure Q6.5A is pregnant with her first child. Both her family and her husband have a sister who is affected by an autosomal recessive trait as shown.

**a.** What is the probability that their child will not be affected by this trait?

**b.** A geneticist uses RFLP analysis (described in Tool Box 3.1), in combination with a polar body assay, to determine if their child is likely to be affected by this trait. In this analysis, the size of the band on the gel represents a chromosome that has either the "normal" allele or the "disease" allele. The gel is shown in Figure Q6.5B, with each lane of the gel showing the results for one member of her family. The lane labeled "PB" is the analysis of the first polar body. Based on this analysis, what is the probability that their child will not be affected by this trait?

**Diagram Description:**

- **Figure Q6.5A** (Pedigree Diagram): 
  - A family tree diagram showing generations I and II.
  - Members are represented by squares (males) and circles (females).
  - Shaded symbols indicate individuals affected by the trait.
  - I-1 and I-4 are unaffected, while I-2 and II-1 are affected.
  - II-2 and her partner are not shaded but connected to the affected sibling II-4.

- **Figure Q6.5B** (Gel Electrophoresis Diagram): 
  - Shows lanes corresponding to members I-1, I-2, II-1, II-2, and PB.
  - Each lane displays bands that differ in size, indicating the presence of "normal" or "disease" alleles.
  - The analysis for the first polar body (PB) shows two distinct bands similar to those of II-2, suggesting allele distribution relevant for predicting the child’s genetic outcome.
Transcribed Image Text:**Looking back 6.17** The woman II-2 shown in the pedigree in Figure Q6.5A is pregnant with her first child. Both her family and her husband have a sister who is affected by an autosomal recessive trait as shown. **a.** What is the probability that their child will not be affected by this trait? **b.** A geneticist uses RFLP analysis (described in Tool Box 3.1), in combination with a polar body assay, to determine if their child is likely to be affected by this trait. In this analysis, the size of the band on the gel represents a chromosome that has either the "normal" allele or the "disease" allele. The gel is shown in Figure Q6.5B, with each lane of the gel showing the results for one member of her family. The lane labeled "PB" is the analysis of the first polar body. Based on this analysis, what is the probability that their child will not be affected by this trait? **Diagram Description:** - **Figure Q6.5A** (Pedigree Diagram): - A family tree diagram showing generations I and II. - Members are represented by squares (males) and circles (females). - Shaded symbols indicate individuals affected by the trait. - I-1 and I-4 are unaffected, while I-2 and II-1 are affected. - II-2 and her partner are not shaded but connected to the affected sibling II-4. - **Figure Q6.5B** (Gel Electrophoresis Diagram): - Shows lanes corresponding to members I-1, I-2, II-1, II-2, and PB. - Each lane displays bands that differ in size, indicating the presence of "normal" or "disease" alleles. - The analysis for the first polar body (PB) shows two distinct bands similar to those of II-2, suggesting allele distribution relevant for predicting the child’s genetic outcome.
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