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A. The following shows a DNA fragment with a 2. operator. Circled bases make direct contacts with repressor protein el and the underlined base pairs are repositioned upon cl binding suh that they become more exposed to the surrounding environment. Describe in detail how you would perform a dimethyl sulfate (DMS) footprinting assay on this fragment and show the expected results (on a gel) with and without cl. (Note: the fragment 25 base-pairs in length). You can assume that the top and bottom strands are individually radiolabeled to start with (i.e. you don't have to explain how you would phosphorylate them with radioactive ATP). GTCACGIOTGACGIACGTACGTACGICATACOTGAC CAGTOCATACTGCATOCATGO®I@CAGTOIGCAOTG

A. The following shows a DNA fragment with a 2. operator. Circled bases make direct contacts with repressor protein el and the underlined base pairs are repositioned upon cl binding suh that they become more exposed to the surrounding environment. Describe in detail how you would perform a dimethyl sulfate (DMS) footprinting assay on this fragment and show the expected results (on a gel) with and without cl. (Note: the fragment 25 base-pairs in length). You can assume that the top and bottom strands are individually radiolabeled to start with (i.e. you don't have to explain how you would phosphorylate them with radioactive ATP). GTCACGIOTGACGIACGTACGTACGICATACOTGAC CAGTOCATACTGCATOCATGO®I@CAGTOIGCAOTG

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A. The following shows a DNA fragment with a 2. operator. Circled bases make direct contacte with repressor protein cl and the underlined base pairs are repositioned upon cl binding such that they become more exposed to the surrounding environment. Describe in detail how you would perform a dimethyl sulfate (DMS) footprinting assay on this fragment and show the expected results (on a gel) with and without cl. (Note: the fragment 25 base-pairs in length). You can assume that the top and bottom strands are individually radiolabeled to start with (i.e. you don't have to explain how you would phosphorylate them with radioactive ATP). GTCACGIOTGACGIOCGTACGTACGICATACOTGAC CAGTOCATAOTGCAT@CATGOOIGCAGIAICCAOTG 个个
Transcribed Image Text:A. The following shows a DNA fragment with a 2. operator. Circled bases make direct contacte with repressor protein cl and the underlined base pairs are repositioned upon cl binding such that they become more exposed to the surrounding environment. Describe in detail how you would perform a dimethyl sulfate (DMS) footprinting assay on this fragment and show the expected results (on a gel) with and without cl. (Note: the fragment 25 base-pairs in length). You can assume that the top and bottom strands are individually radiolabeled to start with (i.e. you don't have to explain how you would phosphorylate them with radioactive ATP). GTCACGIOTGACGIOCGTACGTACGICATACOTGAC CAGTOCATAOTGCAT@CATGOOIGCAGIAICCAOTG 个个
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