A water sample is analysed for coliform organisms by the multiple-tube fermentation method. The results of confirmed test are as follows: Sample Size (m/) Number of positive result out of 5 tubes 5 Number of negative results out of 5 tubes 0 3 0.01 0.001 0.001 1 4 The most probable number (MPN) of coliform organisms for the above results is to be obtained using the following MPN index. 2 MPN Index for various Combination of Positive Results when Five Tubes used per dilution of 10.0 m/, 1.0 m/ and 0.1 m/ Combination of positive tubes MPN index per 100 m/ 0-2-4 1-3-5 4-2-0 5-3-1 11 19 22 110
A water sample is analysed for coliform organisms by the multiple-tube fermentation method. The results of confirmed test are as follows: Sample Size (m/) Number of positive result out of 5 tubes 5 Number of negative results out of 5 tubes 0 3 0.01 0.001 0.001 1 4 The most probable number (MPN) of coliform organisms for the above results is to be obtained using the following MPN index. 2 MPN Index for various Combination of Positive Results when Five Tubes used per dilution of 10.0 m/, 1.0 m/ and 0.1 m/ Combination of positive tubes MPN index per 100 m/ 0-2-4 1-3-5 4-2-0 5-3-1 11 19 22 110
Chapter2: Loads On Structures
Section: Chapter Questions
Problem 1P
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Question
Determine the MPN of coliform organism per 100 ml ?

Transcribed Image Text:A water sample is analysed for coliform organisms by the multiple-tube fermentation
method. The results of confirmed test are as follows:
Sample Size (m/)
0.01
Number of positive
result out of 5 tubes
5
0.001
3
0.001
1
4
The most probable number (MPN) of coliform organisms for the above results is to be
obtained using the following MPN index.
Number of negative
results out of 5 tubes
0
0-2-4
1-3-5
4-2-0
5-3-1
MPN Index for various Combination of Positive Results
when Five Tubes used per dilution of 10.0 m/, 1.0 m/ and 0.1 m/
Combination of positive tubes MPN index per 100 m/
2
11
19
22
110
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