A urine test on a 1-month-old baby shows a positive copper reduction test with a negative oxidase test. How should these results be reported and what is their significance?
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A urine test on a 1-month-old baby shows a positive copper reduction test with a negative oxidase test. How should these results be reported and what is their significance?
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- What are the clinical importance of the catalase and oxidase tests?Reports have indicated that a metabolic byproduct of Vitamin B6 produces 4-pyridoxic acid.(1) This metabolite has fluorescent properties to could “cross-talk” with quinine (i.e., increase the fluorescent signal used for quinine and result in artificially high quinine values).(2) What techniques could we employ to separate 4-pyridoxic acid and quinine in the urine sample? This is a BSL-2 lab.Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patient
- A treatment for hyperammonemia (excess NH4 + in the blood) is to administer pyruvate. What two enzymes are necessary to detoxify the ammonium ion in the presence of pyruvate? What is the product?Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies - +++ Benedict's Reagent +++ +++ +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (b) whether this deduction is confirmed by the detection of ketone bodies and sugar in the urine samples from that patient ||Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.
- Can you help me to find procedure for measuring the activity of the acid phosphatase of the prostrate gland in human blood serum ?after a 5 g onion sample is divided into two equal parts, heat treatment to one of these partsit is being implemented. Then both heat-treated and non-treated parts are 10 times their weightit is ground in an environment containing as much buffer and the determination of pyruvate in the supernatants obtainedit's being done.a) As a result of measurements taken at 520 nm; absorbance value for the heat-treated sample;it was found that the absorbance value in the sample that was not heat treated was 0.123 and the absorbance value in the sample that was not heat treated was 0.520.The equation of the calibration accuracy for pyruvate is that y = 0.1367x – 0.001 (nmol/mL)according to the onion sample; a) independent of alinase activity; b) dependent on alinase activityand c) calculate the total pyruvate amounts in terms of nmol/g of onion.b) How is there a relationship between the activity of the enzyme allinase in onions and the amount of pyruvate?Dec,please explain.Explain why deaminase activity must be determined immediately followingthe addition of ferric chloride.
- Which test is used to differentiate between fermentative and oxidative metabolism? Describe the different results that may be observed with this test.If a sample is composed of a mixture of maltose, maltodextrin, and glucose, which of the following is expected? Adding alkaline CuSO4 to the solution will turn the solution into orange with some dark red precipitate. Performing Molisch Test on the sample will give a negative result. Reaction of the sample with phenylhydrazine will give a solution with yellow precipitate. Furthermore, inspection of the precipitate under the microscope will show cotton ball shaped crystals. . The Osazone Test on the sample will give a yellow solution with no visible precipitate.Absorbance is directly proportional to glucose concentration for both routine clinical glucose spectrophotometry methods: glucose oxidase method and the hexokinase method. For each one, name the final product measured in each test reaction, which is proportional to the sample glucose concentration. A.) glucose oxidase: B.) hexokinase: