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A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase gene. Before the gene could be cloned for further work, the genome of this bacteria should first be extracted. (i) State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. (ii) After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified.

A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase gene. Before the gene could be cloned for further work, the genome of this bacteria should first be extracted. (i) State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. (ii) After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase gene. Before the gene could be cloned for further work, the genome of this bacteria should first be extracted. (i) State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified. (ii)
Transcribed Image Text:A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase gene. Before the gene could be cloned for further work, the genome of this bacteria should first be extracted. (i) State FOUR (4) main methods you may use to lyse the cells prior to DNA isolation. Give ONE (1) example of each method. After the genome is obtained, explain in detail how the amylase gene could be cloned into Escherichia coli competent cells to be amplified. (ii)
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