A student made HPLC analysis to find unknown concentration of Vitamin C sample. She prepared 800 ppm reference solution with Vitamin C. She took 1 mL from this solution into 25 mL volumetric flask, completed with solvent, filtered, and analyzed. She found peak area as 172,50 mAu*s. Then she analyzed unknown concentration sample and she found peak area as 134,76 mAu*s. What is the concentration of unknown sample? 624.97 ppm 62.49 ppm 29.06 ppm 25.00 ppm

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A student made HPLC analysis to find unknown concentration of Vitamin C
sample. She prepared 800 ppm reference solution with Vitamin C. She took
1 mL from this solution into 25 mL volumetric flask, completed with
solvent, filtered, and analyzed. She found peak area as 172,50 mAu*s.
Then she analyzed unknown concentration sample and she found peak
area as 134,76 mAu*s. What is the concentration of unknown sample?
624.97 ppm
62.49 ppm
29.06 ppm
25.00 ppm
Transcribed Image Text:A student made HPLC analysis to find unknown concentration of Vitamin C sample. She prepared 800 ppm reference solution with Vitamin C. She took 1 mL from this solution into 25 mL volumetric flask, completed with solvent, filtered, and analyzed. She found peak area as 172,50 mAu*s. Then she analyzed unknown concentration sample and she found peak area as 134,76 mAu*s. What is the concentration of unknown sample? 624.97 ppm 62.49 ppm 29.06 ppm 25.00 ppm
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