A student is preparing 250 mL of a solution of 155.0 mM NaCl in 50.0 mM phosphate pH 6.4. The student has stock solutions of 0.850 M NaCl and 0.400 M phosphate pH 6.4. Calculate the volume of phosphate required to make the solution (in mL).
A student is preparing 250 mL of a solution of 155.0 mM NaCl in 50.0 mM phosphate pH 6.4. The student has stock solutions of 0.850 M NaCl and 0.400 M phosphate pH 6.4. Calculate the volume of phosphate required to make the solution (in mL).
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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A student is preparing 250 mL of a solution of 155.0 mM NaCl in 50.0 mM phosphate pH 6.4. The student has stock solutions of 0.850 M NaCl and 0.400 M phosphate pH 6.4. Calculate the volume of phosphate required to make the solution (in mL).
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Step 1
First, we have to find the moles (or millimoles, mmol) of phosphate present in the 250 mL of 50.0 mM phosphate buffer.
Then we can find the volume of the stock phosphate (0.400 M) required to prepare the above buffer solution.
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