A researcher treated a sample (660 mg) of an oligomeric protein of M, 132,000 with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. He then completely hydrolyzed the peptide bonds of the protein by heating it with concentrated HCl. The hydrolysate was found to contain 5.5 mg of the compound, DNP-valine, shown here. сH, сн, NO, CH O,N- - NH-Ć-COOH H 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. How can this information help determine the number of polypeptide chains in an oligomeric protein?

A researcher treated a sample (660 mg) of an oligomeric protein of M, 132,000 with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) under slightly alkaline conditions until the chemical reaction was complete. He then completely hydrolyzed the peptide bonds of the protein by heating it with concentrated HCl. The hydrolysate was found to contain 5.5 mg of the compound, DNP-valine, shown here. сH, сн, NO, CH O,N- - NH-Ć-COOH H 2,4-Dinitrophenyl derivatives of the a-amino groups of other amino acids could not be found. How can this information help determine the number of polypeptide chains in an oligomeric protein?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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