A researcher is investigating the effect of temperature on the growth of a bacterial culture. They inoculate 3 broth cultures with equal starting amounts of bacteria and place one each in incubators set at 25°C, 35°C and 45°C for 24 hours under standard oxygen concentrations. After 24 hours, growth is assessed using a spectrophotometer to measure turbidity. A) Explain why it is important to keep the atmospheric conditions, duration, and other parameters aside from temperature identical for these cultures. B) What is another method to determine the extent of microbial growth in this scenario? C) Hypothesize which culture would be expected to display the greatest turbidity for a mesophile. I
A researcher is investigating the effect of temperature on the growth of a bacterial culture. They inoculate 3 broth cultures with equal starting amounts of bacteria and place one each in incubators set at 25°C, 35°C and 45°C for 24 hours under standard oxygen concentrations. After 24 hours, growth is assessed using a spectrophotometer to measure turbidity. A) Explain why it is important to keep the atmospheric conditions, duration, and other parameters aside from temperature identical for these cultures. B) What is another method to determine the extent of microbial growth in this scenario? C) Hypothesize which culture would be expected to display the greatest turbidity for a mesophile. I
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:A researcher is investigating the effect of temperature on the growth of a bacterial culture. They
inoculate 3 broth cultures with equal starting amounts of bacteria and place one each in
incubators set at 25°C, 35°C and 45°C for 24 hours under standard oxygen concentrations. After
24 hours, growth is assessed using a spectrophotometer to measure turbidity.
A) Explain why it is important to keep the atmospheric conditions, duration, and other parameters
aside from temperature identical for these cultures.
B) What is another method to determine the extent of microbial growth in this scenario?
C) Hypothesize which culture would be expected to display the greatest turbidity for a mesophile.
I
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