please answer a A production strain of L-threonine was derived from fermentation of Escherichia coli (E.Coli).A biochemical engineer has determined the cell growth, product formation and substrateconsumption. The data were tabulated in Table 1. Moreover, the dissolved oxygenconcentration was measured to estimate the oxygen uptake rate and volumetric oxygentransfer coefficient (k, · a) in this system.a) Estimate the volumetric oxygen transfer coefficient (kL · a) for the fermentation process.The critical oxygen concentration must be above 10% of the saturation level. Given thatthe yield of oxygen coefficient is 0.45 g cell/g O2 and oxygen solubility is 7.5 mg/L at cellconcentration of 60 g/L.b) Determine the maximum concentration of E.coli that can be sustained in the fermenterunder steady-state condition if the specific rate of oxygen consumption in (a) is doubled.Conclude your finding.Table 1: Cell growth, substrate concentration and product formation data.TimeE.ColiDissolvedОхудen(mg/L)GlucoseThreonine(h)concentrationconcentrationConcentration(g/L)(g/L)(g/L)1457.872437.76126.4116.3818153634.78243030292.99304522571.98365416762.8942589.914.484860986.4754600.2100760600.11017.01 Useful equationkL a[C* – C¿]The rate of oxygen transfer, OTRThe oxygen uptake rate,HgXOUR =902 *whereis the oxygen transfer coefficient (cm/hr)is the gas-liquid interfacial area (cm?/cm³)kLаkL·a is the volumetric oxygen transfer coefficientC*is the saturated DO concentration (mg/L)is the actual DO concentration in the broth (mg/L)is the specific rate of oxygen consumption (mg Oz/g dw cells.hr)CL902is specific growth rate (hr')Hgis the yield coefficient on oxygen (g dw cells/g O2)Xis cell concentration (g dw cell/L)

The objective of this question is to L-threonine production by Escherichia coli (Ecoli)…

A production strain of L-threonine was derived from fermentation of Escherichia coli (E. Coli). A biochemical engineer has determined the cell growth, product formation and substrate consumption. The data were tabulated in Table 1. Moreover, the dissolved oxygen concentration was measured to estimate the oxygen uptake rate and volumetric oxygen transfer coefficient (ka) in this system. a) Estimate the volumetric oxygen transfer coefficient (k, a) for the fermentation process. The critical oxygen concentration must be above 10% of the saturation level. Given that the yield of oxygen coefficient is 0.45 g cell/g O2 and oxygen solubility is 7.5 mg/L at cell concentration of 60 g/L.

A production strain of L-threonine was derived from fermentation of Escherichia coli (E. Coli). A biochemical engineer has determined the cell growth, product formation and substrate consumption. The data were tabulated in Table 1. Moreover, the dissolved oxygen concentration was measured to estimate the oxygen uptake rate and volumetric oxygen transfer coefficient (ka) in this system. a) Estimate the volumetric oxygen transfer coefficient (k, a) for the fermentation process. The critical oxygen concentration must be above 10% of the saturation level. Given that the yield of oxygen coefficient is 0.45 g cell/g O2 and oxygen solubility is 7.5 mg/L at cell concentration of 60 g/L.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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