A patient visits an urgent care center with complaints of a stiff neck and headache. While collecting the medical history, the urgent care physician assistant (PA) discovers that the patient had a lumbar tap 5 days ago. The patient’s current temperature is 101°F. The PA is concerned that the patient may have contracted meningitis. The PA orders laboratory analysis immediately, including a CBC that uses flow cytometry technology. 1. The laboratory is equipped to perform flow cytometry. Which of the following descriptions best describes this testing technique? A. Light is passed through a solution and scattered at an angle. A detection device collects the scattered light. The angle of the scatter corresponds to the concentration of the solution. B. Light is passed through a solution and scattered in a linear fashion. A detection device collects the scattered light. The amount of light scatter is proportional to the size, shape, and concentration of molecules present in solution. C. Blood is aspirated into an instrument where cells pass one by one through a counting chamber. A laser shines on the passing cells. The light scattered when the laser hits each cell is measured to determine cell characteristics. 2. The size of each cell is measured by forward light scatter. Many of the cells flowing through the instrument chamber produced increased forward light scatter. What does this finding tell you about the cells in this patient’s sample? A. The cell are large B. The cell are small
A patient visits an urgent care center with complaints of a stiff neck and headache. While collecting the medical history, the urgent care physician assistant (PA) discovers that the patient had a lumbar tap 5 days ago. The patient’s current temperature is 101°F. The PA is concerned that the patient may have contracted meningitis. The PA orders laboratory analysis immediately, including a CBC that uses flow cytometry technology.
1. The laboratory is equipped to perform flow cytometry. Which of the following descriptions best describes this testing technique?
A. Light is passed through a solution and scattered at an angle. A detection device collects the scattered light. The angle of the scatter corresponds to the concentration of the solution.
B. Light is passed through a solution and scattered in a linear fashion. A detection device collects the scattered light. The amount of light scatter is proportional to the size, shape, and concentration of molecules present in solution.
C. Blood is aspirated into an instrument where cells pass one by one through a counting chamber. A laser shines on the passing cells. The light scattered when the laser hits each cell is measured to determine cell characteristics.
2. The size of each cell is measured by forward light scatter. Many of the cells flowing through the instrument chamber produced increased forward light scatter. What does this finding tell you about the cells in this patient’s sample?
A. The cell are large
B. The cell are small
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