A medical lab reports a blood alcohol result of 12 mg/dl. Convert to its equivalent value in common forensic units: A) 0.12% v/v B) 0.12% w/v C) 0.012 M D) 0.012% v/v E) 0.12 N OF) 0.012 N G 0.012% w/v H) 0.12 M

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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A medical lab reports a blood alcohol result of 12 mg/dl. Convert to its equivalent value in common forensic units:
OA) 0.12% V/V
B) 0.12% w/v
C) 0.012 M
D) 0.012% v/v
E) 0.12 N
F) 0.012 N
G 0.012% w/v
OH) 0.12 M
Transcribed Image Text:A medical lab reports a blood alcohol result of 12 mg/dl. Convert to its equivalent value in common forensic units: OA) 0.12% V/V B) 0.12% w/v C) 0.012 M D) 0.012% v/v E) 0.12 N F) 0.012 N G 0.012% w/v OH) 0.12 M
→ Question 13 Retaken
A published protocol of extracting GHB (gamma-hydroxybutyric acid): "To aliquots (1 mL) of the sample (blood and urine standards,
controls, and unknowns), 50 µL of the internal standard working solution, DEG (diethyleneglycol), was added, followed by 250 µL of
cold 0.1N H₂SO4. The samples were vortex mixed. Ethyl acetate (6 mL) was added, and the compounds were extracted for 10 min on a
rotating mixer, then centrifuged at 2500 rpm for 5 min. The organic layer was transferred to a clean extraction tube, and the aqueous layer
was re-extracted with an additional 6 mL of ethyl acetate for 10 min. Following centrifugation, the organic layer was transferred." The
DEG and GHB were then actually derivatized to their di-TMS adducts for GCMS analysis.
Why was DEG was used as the internal standard?
A) Because it readily derivatizes GHB.
B) Because DEG and GHB are not soluble in each other.
C) Because it is chemically similar to GHB and their di-TMS derivatives are also chemically similar.
D) Because they go to different layers during liquid-liquid extraction.
Transcribed Image Text:→ Question 13 Retaken A published protocol of extracting GHB (gamma-hydroxybutyric acid): "To aliquots (1 mL) of the sample (blood and urine standards, controls, and unknowns), 50 µL of the internal standard working solution, DEG (diethyleneglycol), was added, followed by 250 µL of cold 0.1N H₂SO4. The samples were vortex mixed. Ethyl acetate (6 mL) was added, and the compounds were extracted for 10 min on a rotating mixer, then centrifuged at 2500 rpm for 5 min. The organic layer was transferred to a clean extraction tube, and the aqueous layer was re-extracted with an additional 6 mL of ethyl acetate for 10 min. Following centrifugation, the organic layer was transferred." The DEG and GHB were then actually derivatized to their di-TMS adducts for GCMS analysis. Why was DEG was used as the internal standard? A) Because it readily derivatizes GHB. B) Because DEG and GHB are not soluble in each other. C) Because it is chemically similar to GHB and their di-TMS derivatives are also chemically similar. D) Because they go to different layers during liquid-liquid extraction.
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