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A lab conducts an experiment to identify an RNA/protein interaction. This is performed similarly to an immunoprecipitation (IP) in that an antibody is used to immunoprecipitate a protein of interest, after which they identify whether a particular RNA is associated with that protein. Specifically, the lab is looking to study a potential interaction between the FXR1 protein and the miR301a-3p RNA. They start with cells that express both FXR1 and miR301a-3p and perform two IP's, one with an IgG antibody (a control antibody that does not bind to FXR1) and a second with an anti-FXR1 antibody. They then detect the amount of the miR301a-3p RNA that is associated with the immunoprecipitate. Which of the following techniques could be used to determine the amount of miR301a-3p RNA in the immunoprecipitate? Select all that apply. O RNA Seq O Microarray O Quantitative RT-PCR EMSA

A lab conducts an experiment to identify an RNA/protein interaction. This is performed similarly to an immunoprecipitation (IP) in that an antibody is used to immunoprecipitate a protein of interest, after which they identify whether a particular RNA is associated with that protein. Specifically, the lab is looking to study a potential interaction between the FXR1 protein and the miR301a-3p RNA. They start with cells that express both FXR1 and miR301a-3p and perform two IP's, one with an IgG antibody (a control antibody that does not bind to FXR1) and a second with an anti-FXR1 antibody. They then detect the amount of the miR301a-3p RNA that is associated with the immunoprecipitate. Which of the following techniques could be used to determine the amount of miR301a-3p RNA in the immunoprecipitate? Select all that apply. O RNA Seq O Microarray O Quantitative RT-PCR EMSA

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A lab conducts an experiment to identify an RNA/protein interaction. This is performed similarly to an immunoprecipitation (IP) in that an antibody is used to immunoprecipitate a protein of interest, after which they identify whether a particular RNA is associated with that protein. Specifically, the lab is looking to study a potential interaction between the FXR1 protein and the miR301a-3p RNA. They start with cells that express both FXR1 and miR301a-3p and perform two IP's, one with an IgG antibody (a control antibody that does not bind to FXR1) and a second with an anti-FXR1 antibody. They then detect the amount of the miR301a-3p RNA that is associated with the immunoprecipitate. Which of the following techniques could be used to determine the amount of miR301a-3p RNA in the immunoprecipitate? Select all that apply. RNA Seq Microarray O Quantitative RT-PCR O EMSA
Transcribed Image Text:A lab conducts an experiment to identify an RNA/protein interaction. This is performed similarly to an immunoprecipitation (IP) in that an antibody is used to immunoprecipitate a protein of interest, after which they identify whether a particular RNA is associated with that protein. Specifically, the lab is looking to study a potential interaction between the FXR1 protein and the miR301a-3p RNA. They start with cells that express both FXR1 and miR301a-3p and perform two IP's, one with an IgG antibody (a control antibody that does not bind to FXR1) and a second with an anti-FXR1 antibody. They then detect the amount of the miR301a-3p RNA that is associated with the immunoprecipitate. Which of the following techniques could be used to determine the amount of miR301a-3p RNA in the immunoprecipitate? Select all that apply. RNA Seq Microarray O Quantitative RT-PCR O EMSA
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