A functional protein that is approximately 110 kDa in size has all its cysteine residues joined with disulfide bonds. It has one cysteine residue per 100 amino acids. When heated at 85°C it lost activity, but when it was allowed to caol, the activity was restored? Please answer the 2. following questions: a) How many disulfide bonds the protein has? Show how you came up with the answer. b) What is the molecular basis for the protein behavior in restoring activity?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A functional protein that is approximately 110 kDa in size has all its cysteine residues joined
with disulfide bonds. It has one cysteine residue per 100 amino acids. When heated at 85°C it
lost activity, but when it was allowed to caol, the activity was restored? Please answer the
2.
following questions:
a) How many disulfide bonds the protein has? Show how you came up with the answer.
b) What is the molecular basis for the protein behavior in restoring activity?
Transcribed Image Text:A functional protein that is approximately 110 kDa in size has all its cysteine residues joined with disulfide bonds. It has one cysteine residue per 100 amino acids. When heated at 85°C it lost activity, but when it was allowed to caol, the activity was restored? Please answer the 2. following questions: a) How many disulfide bonds the protein has? Show how you came up with the answer. b) What is the molecular basis for the protein behavior in restoring activity?
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