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A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which of the following media would allow growth of bacteria containing a plasmid with eukaryotic gene? O the nutrient broth and the tetracycline broth only the nutrient broth and the ampicillin broth only the nutrient broth only

A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which of the following media would allow growth of bacteria containing a plasmid with eukaryotic gene? O the nutrient broth and the tetracycline broth only the nutrient broth and the ampicillin broth only the nutrient broth only

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which of the following media would allow growth of bacteria containing a plasmid with eukaryotic gene? O the nutrient broth and the tetracycline broth only the nutrient broth and the ampicillin broth only the nutrient broth only O the nutrient broth, the ampicillin broth, and the tetracycline broth.
Transcribed Image Text:A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which of the following media would allow growth of bacteria containing a plasmid with eukaryotic gene? O the nutrient broth and the tetracycline broth only the nutrient broth and the ampicillin broth only the nutrient broth only O the nutrient broth, the ampicillin broth, and the tetracycline broth.
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