A calibration curve for the colorimetric determination of phosphorous in urine is prepared by reacting standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce the characteristic blue color. The measured absorbance A is plotted against the concentration of phosphorus. From the following data, determine the linear least-squares equation and calculate ppm P in the urine sample: ppm P A 1.00 0.205 2.00 0.410 3.00 0.615 4.00 0.820 Urine sample 0.625

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A calibration curve for the colorimetric determination of phosphorous in urine is prepared by reacting
standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce
the characteristic blue color. The measured absorbance A is plotted against the concentration of phosphorus. From
the following data, determine the linear least-squares equation and calculate ppm P in the urine sample:
ppm P
1.00
A
0.205
2.00
0.410
3.00
0.615
4.00
0.820
Urine sample
0.625
2.
Transcribed Image Text:A calibration curve for the colorimetric determination of phosphorous in urine is prepared by reacting standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce the characteristic blue color. The measured absorbance A is plotted against the concentration of phosphorus. From the following data, determine the linear least-squares equation and calculate ppm P in the urine sample: ppm P 1.00 A 0.205 2.00 0.410 3.00 0.615 4.00 0.820 Urine sample 0.625 2.
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