please explain the figuer abA-T (FT169)VectorIR (10 Gy):WTWortmannin:IR (10 Gy):cap T2609aDNA-PKcs1 2 31 2 3Ku8o SIANA:IR (10 Gy):aKu80apT2609aDNA-PKcsap5966-SMC1aSMC11 2 3d 121.0e1412100.8-0.6-8-6-0.4 -0.20-2 31Lanes in Ku80 Westem2Lanes in pT2609 Western3Figure 3. Characterization of Thr2609 phosphorylation in vivo. (a)Inhibition of Thr2609 phosphorylation by wortmannin. HeLa cellswere preincubated with DMSO (dimethyl sulfoxide; lanes 1,2) or 20µM wortmannin (lane 3) for 30 min before treatment with 10 Gy ofionizing radiation (IR; lanes 2,3). Nuclear extracts were prepared 30min after IR and subsequently analyzed for Thr2609 phosphoryla-tion (top) and with 25-4 monoclonal (bottom). (b) Phosphorylation ofThr2609 is ATM-independent. A-T cells (FT169) complementedwith vector (lanes 1,2) or full-length wild-type ATM CDNA (lanes3,4) were treated with 10 Gy of IR. Nuclear extracts were analyzedfor Thr2609 phosphorylation (top) and DNA-PKcs levels (bottom).(c) Phosphorylation of Thr2609 is Ku-dependent. HeLa cells weremock-transfected (lanes 1,2) or transfected with a short interferingKu80 RNA (lane 3) for 96 h before irradiation with 10 Gy of IR.Nuclear extracts were analyzed by Western blotting with the indi-cated antibodies. (d) Quantification of Ku80 Western signal from c.Values reported are relative to the signal observed in lane 1 andassigned an arbitrary value of one. (e) Quantification of pT2609Absignal from c. Values reported are relative to the signal observed inlane 1.

Name: please explain the figuer abA-T (FT169)VectorIR (10 Gy):WTWortmannin:I
Uploaded: 2024-03-20T06:42:55.000Z
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Description: please explain the figuer abA-T (FT169)VectorIR (10 Gy):WTWortmannin:IR (10 Gy):cap T2609aDNA-PKcs1 2 31 2 3Ku8o SIANA:IR (10 Gy):aKu80apT2609aDNA-PKcsap5966-SMC1aSMC11 2 3d 121.0e1412100.8-0.6-8-6-0.4 -0.20-2 31Lanes in Ku80 Westem2Lanes in pT2609 W