Question 5 Electromagnetic phenomena and spectroscopy.(a) A medical researcher is using the uniform electric field (E = 2400 NC-¹) generatedby an electrophoresis setup to isolate a charged protein of interest (q = +160e) from anuncharged patient sample. As shown in panel (A), when the patient sample is loadedbetween the positive and negative terminal, the charged protein of interest migrates 12cm toward the negative terminal.(A)(B)€€€(ii)(iii)(iv)(c)+160e-0(1)(ii)(iii)12 cm= protein of interest= patient sampleIntensity (a.u.)10080604020ol450Excitationspectrum(b) Migration of the charged protein of interest was monitored by staining the patientsample with a fluorescent dye that was specific for this biological molecule. The excitationand emission spectrum of this fluorescent dye are presented in panel (B).(1)Emissionspectrum500 550 600Wavelength (nm)How much work has the electric field done on the protein of interest?What is the protein of interest's change in electric potential energy?If the medical researcher wanted the charged protein of interest to migrate furtheraway from the uncharged patient sample, how could they achieve this?What is the strength of the massspectrometer's magnetic field?Is the magnetic field vector pointing into thepage or out of the page?What is the mass of component A?650What wavelength of light (A) could be used to excite the fluorescent dye highlightingthe protein of interest within the electrophoresis gel?What wavelength of light (A) could be used to detect the resulting fluorescence?Calculate the photon energy associated with the wavelength of light (A) that yousuggest could excite the fluorescent dye.Briefly describe why the emission spectrum of the fluorescent dye in panel (B)occurs at longer wavelengths of light (A) compared to its excitation spectrum.The extracted protein of interest is then subjected to mass spectrometry forcharacterisation. As depicted in panel (C), when the protein enters the magnetic field ofthe mass spectrometer with a velocity of 9 x 104 ms-1, two singly ionised components (Aand B) are detected 8 cm and 10 cm to the left of the point of entry. The mass ofcomponent B is reported to be 7 x 10-23 kg.(C)2 cmA B00248 cm Protein ofinterest

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Answered: (a) A medical researcher is using the…

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