A 20 mol% glutaraldehyde, 80 mol% water solution can be used for protein precipitation, a key step in enzyme crosslinking. Relevant data: you must use for full credit! ∆?vap glutaraldehyde = 40,160 J/mol ∆?fus glutaraldehyde =13,280 J/mol Tvap glutaraldehyde = 374 K Tfus glutaraldehyde = 267 K b) Calculate the freezing temperature of the solution (assume this behaves as an ideal solution). Show all work. Does your answer make rational sense? Why or why not? c) On the same plot of chemical potential versus temperature, draw 4 traces: pure solid glutaraldehyde, pure liquid glutaraldehyde, pure gaseous glutaraldehyde, an
A 20 mol% glutaraldehyde, 80 mol% water solution can be used for protein precipitation, a key step in enzyme crosslinking. Relevant data: you must use for full credit!
∆?vap glutaraldehyde = 40,160 J/mol ∆?fus glutaraldehyde =13,280 J/mol
Tvap glutaraldehyde = 374 K Tfus glutaraldehyde = 267 K
b) Calculate the freezing temperature of the solution (assume this behaves as an ideal solution). Show all work. Does your answer make rational sense? Why or why not?
c) On the same plot of chemical potential versus temperature, draw 4 traces: pure solid glutaraldehyde, pure liquid glutaraldehyde, pure gaseous glutaraldehyde, and glutaraldehyde in solution. Clearly label the melting and boiling points for the pure substance (given) vs. the solution with those you calculated in parts a and b.
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