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A 1.0 ml protein solution in 0.500 M TRIS buffer, pH 7 with 0.250 M NaCl was dialyzed overnight against 500 mL of 0.500 M Tris, pH 7. What was the concentration of NaCl in the protein solution after equilibration?

A 1.0 ml protein solution in 0.500 M TRIS buffer, pH 7 with 0.250 M NaCl was dialyzed overnight against 500 mL of 0.500 M Tris, pH 7. What was the concentration of NaCl in the protein solution after equilibration?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Laboratory methods used to isolate, purify and characterize proteins. Dialysis is commonly used to remove small molecules and ions from protein solutions A 1.0 ml protein solution in 0.500 M TRIS buffer, pH 7 with 0.250 M NaCl was dialyzed overnight against 500 mL of 0.500 M Tris, pH 7. What was the concentration of NaCl in the protein solution after equilibration? Dialysis bag Concentrated solution Buffer At start of dialysis At equilibrium Figure 32 echemi Seventh en e2012weeman and Compary Dialyzing tubing comes in various pore sizes
Transcribed Image Text:Laboratory methods used to isolate, purify and characterize proteins. Dialysis is commonly used to remove small molecules and ions from protein solutions A 1.0 ml protein solution in 0.500 M TRIS buffer, pH 7 with 0.250 M NaCl was dialyzed overnight against 500 mL of 0.500 M Tris, pH 7. What was the concentration of NaCl in the protein solution after equilibration? Dialysis bag Concentrated solution Buffer At start of dialysis At equilibrium Figure 32 echemi Seventh en e2012weeman and Compary Dialyzing tubing comes in various pore sizes
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