9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions.

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9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL
sample of acetylcholine (a neurotransmitter) with an unknown concentration
and a pH of 7.65. You incubate this sample with the enzyme
acetylcholinesterase to convert all of the acetylcholine to choline and acetic
acid. The acetic acid dissociates to yield acetate and hydrogen ions.
O
CH3
CH3-C-O-CH2-CH2-N–CH3
Acetylcholine
CH3
H₂O
CH3
HỌCH, CH, N– CH3 + CH3-CHO TH
11
CH3
Choline
Acetate
T
4
At the end of the incubation period, you measure the pH again and find that it
has decreased to 6.87. Assuming there was no buffer in the assay mixture,
determine the number of nanomoles of acetylcholine in the original 15 mL
sample.
Transcribed Image Text:9. Measurement of Acetylcholine Levels by pH Changes You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.65. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. O CH3 CH3-C-O-CH2-CH2-N–CH3 Acetylcholine CH3 H₂O CH3 HỌCH, CH, N– CH3 + CH3-CHO TH 11 CH3 Choline Acetate T 4 At the end of the incubation period, you measure the pH again and find that it has decreased to 6.87. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample.
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